Volume 5, Issue 4 (December 2018)
J. Food Qual. Hazards Control 2018, 5(4): 140-145 |
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Nutrition Research Center, Department of Food Science and Technology, Faculty of Nutrition and Food Science, Tabriz University of Medical Sciences, Tabriz, Iran , dehghanp@tbzmed.ac.ir
Abstract: (3404 Views)
Background: In recent years, food-borne outbreaks have been increased by consumption of raw fruits and vegetables contaminated with bacterial pathogens like Salmonella spp. in many countries. This study was designed in order to molecular detection of Salmonella in Ready-to-Eat Vegetable Salad (REVS) consumed in restaurants of Tabriz, North-West of Iran.
Methods: In this cross-sectional study, 90 REVS samples were randomly collected from five different areas of the Tabriz, Iran from February to June 2016. The presence of S. enterica serovar Typhimurium isolates was assessed using real-time polymerase chain reaction technique. Data analysis was performed using SPSS software version 19.0.
Results: Out of 90 REVS samples, 4 (4.44%) were found to be positive with S. enterica serovar Typhimurium. There was no significant difference between S. enetrica serovar Typhimurium prevalence in various areas (p>0.05).
Conclusion: This survey showed that REVS consumed in restaurants of Tabriz, Iran may have public health risk in terms of presence of S. enterica serovar Typhimurium; so, it is required to improve the food safety standards in this area. Next investigations should be done to find the antimicrobial susceptibility of the identified isolates.
DOI: 10.29252/jfqhc.5.4.5
Methods: In this cross-sectional study, 90 REVS samples were randomly collected from five different areas of the Tabriz, Iran from February to June 2016. The presence of S. enterica serovar Typhimurium isolates was assessed using real-time polymerase chain reaction technique. Data analysis was performed using SPSS software version 19.0.
Results: Out of 90 REVS samples, 4 (4.44%) were found to be positive with S. enterica serovar Typhimurium. There was no significant difference between S. enetrica serovar Typhimurium prevalence in various areas (p>0.05).
Conclusion: This survey showed that REVS consumed in restaurants of Tabriz, Iran may have public health risk in terms of presence of S. enterica serovar Typhimurium; so, it is required to improve the food safety standards in this area. Next investigations should be done to find the antimicrobial susceptibility of the identified isolates.
DOI: 10.29252/jfqhc.5.4.5
Type of Study: Original article |
Subject:
Special
Received: 18/05/02 | Accepted: 18/09/23 | Published: 18/12/28
Received: 18/05/02 | Accepted: 18/09/23 | Published: 18/12/28
References
1. Abakari G., Cobbina S.J., Yeleliere E. (2018). Microbial quality of ready-to-eat vegetable salads vended in the central business district of Tamale, Ghana. International Journal of Food Contamination. 5: 3. [DOI:10.1186/s40550-018-0065-2]
2. Akoachere J.F.T.K., Tatsinkou B.F., Nkengfack J.M. (2018). Bacterial and parasitic contaminants of salad vegetables sold in markets in Fako Division, Cameroon and evaluation of hygiene and handling practices of vendors. BMC Research Notes. 11: 100. [DOI:10.1186/s13104-018-3175-2] [PMID] [PMCID]
3. Brandão M.L.L., Almeida D.O., Bispo F.C.P., Bricio S.M.L., Marin V.A., Miagostovich M.P. (2014). Assessment of microbiological contamination of fresh, minimally processed, and ready-to-eat lettuces (Lactuca sativa), Rio de Janeiro State, Brazil. Journal of Food Science. 79: 961-966. [DOI:10.1111/1750-3841.12459] [PMID]
4. Caponigro V., Ventura M., Chiancone I., Amato L., Parente E., Piro F. (2010). Variation of microbial load and visual quality of ready-to-eat salads by vegetable type, season, processor and retailer. Food Microbiology. 27: 1071-1077. [DOI:10.1016/j.fm.2010.07.011] [PMID]
5. De Giusti M., Aurigemma C., Marinelli L., Tufi D., De Medici D., Di Pasquale S., De Vito C., Boccia A. (2010). The evaluation of the microbial safety of fresh ready‐to‐eat vegetables produced by different technologies in Italy. Journal of Applied Microbiology. 109: 996-1006. [DOI:10.1111/j.1365-2672.2010.04727.x] [PMID]
6. Dunkley K.D., Callaway T.R., Chalova V.I., McReynolds J.L., Hume M.E., Dunkley C.S., Kubena L.F., Nisbet D.J., Ricke S.C. (2009). Foodborne Salmonella ecology in the avian gastrointestinal tract. Anaerobe.15: 26-35. [DOI:10.1016/j.anaerobe.2008.05.007] [PMID]
7. Elizaquível P., Gabaldón J.A., Aznar R. (2011). Quantification of Salmonella spp., Listeria monocytogenes and Escherichia coli O157:H7 in non-spiked food products and evaluation of real-time PCR as a diagnostic tool in routine food analysis. Food Control. 22: 158-164. [DOI:10.1016/j.foodcont.2010.05.018]
8. Gurler Z., Pamuk S., Yildirim Y., Ertas N. (2015). The microbiological quality of ready-to-eat salads in Turkey: a focus on Salmonella spp. and Listeria monocytogenes. International Journal of Food Microbiology. 196: 79-83. [DOI:10.1016/j.ijfoodmicro.2014.11.021] [PMID]
9. Hur J., Jawale C., Lee J.H. (2012). Antimicrobial resistance of Salmonella isolated from food animals: a review. Food Research International. 45: 819-830. [DOI:10.1016/j.foodres.2011.05.014]
10. Institute of Standards and Industrial Research of Iran (ISIRI). (2009). Food microbiology– Detection of Salmonella by Polymerase Chain Reaction (PCR). National Standard No. 12638. URL: ttp://standard.isiri.gov.ir/StandardView.aspx?Id=11908. Accessed 5 Mar 2017.
11. Institute of Standards and Industrial Research of Iran (ISIRI). (2014). Microbiology of food and animal feeding stuffs- Horizontal method for the detection of Salmonella spp. National Standard No. 1810. URL: http://standard.isiri.gov.ir/ StandardView.aspx?Id=41860. Accessed 5 Mar 2017.
12. Keithlin J., Sargeant J.M., Thomas M.K., Fazil A. (2015). Systematic review and meta-analysis of the proportion of non-typhoidal Salmonella cases that develop chronic sequelae. Epidemiology and Infection. 143: 1333-1351. [DOI:10.1017/S0950268814002829] [PMID] [PMCID]
13. Khalil R.K., Gomaa M.A., Khalil M.I. (2015). Detection of shiga-toxin producing E. coli (STEC) in leafy greens sold at local retail markets in Alexandria, Egypt. International Journal of Food Microbiology. 197:58-64. [DOI:10.1016/j.ijfoodmicro.2014.12.019] [PMID]
14. Klingbeil D.F., Todd E.C.D., Kuri V. (2016). Microbiological quality of ready-to-eat fresh vegetables and their link to food safety environment and handling practices in restaurants. LWT-Food Science and Technology. 74: 224-233. [DOI:10.1016/j.lwt.2016.07.051]
15. Kochakkhani H., Dehghan P., Mousavi M.H., Sarmadi B. (2016). Occurrence, molecular detection and antibiotic resistance profile of Escherichia coli O157:H7 isolated from ready-to-eat vegetable salads in Iran. Pharmaceutical Sciences. 22: 195-202. [DOI:10.15171/PS.2016.31]
16. Kochakkhani H., Moosavy M.H., Dehghan P. (2017). Isolation and identification of Clostridium difficile from ready-to-eat vegetable salads in restaurants of Tabriz by real-time PCR and determination of the antibiotic resistance pattern. Scientific Journal of Kurdistan University of Medical Sciences. 22: 102-112.
17. Kotzekidou P. (2013). Survey of Listeria monocytogenes, Salmonella spp. and Escherichia coli O157:H7 in raw ingredients and ready-to-eat products by commercial real-time PCR kits. Food Microbiology. 35: 86-91. [DOI:10.1016/j.fm.2013.03.007] [PMID]
18. León B.D., Gomez-Aldapa C.A., Rangel-Vargas E., Vazquez-Barrios E., Castro-Rosas J. (2013). Frequency of indicator bacteria, Salmonella and diarrhoeagenic Escherichia coli pathotypes on ready-to-eat cooked vegetable salads from Mexican restaurants. Letters in Applied Microbiology. 56: 414-420. [DOI:10.1111/lam.12063] [PMID]
19. Little C.L., Gillespie I.A. (2008). Prepared salads and public health. Journal of Applied Microbiology. 105: 1729-1743. [DOI:10.1111/j.1365-2672.2008.03801.x] [PMID]
20. Muinde O.K., Kuria E. (2005). Hygienic and sanitary practices of vendors of street foods in Nairobi, Kenya. African Journal of Food, Agriculture, Nutrition and Development. 5: 1-14.
21. Toe E., Dadié A., Dako E., Loukou G. (2017). Bacteriological quality and risk factors for contamination of raw mixed vegetable salads served in collective catering in Abidjan (Ivory Coast). Advances in Microbiology. 7: 405-419. [DOI:10.4236/aim.2017.76033]
22. Vojkovska H., Myskova P., Gelbícova T., Skockova A., Kolackova I., Karpískova R. (2017). Occurrence and characterization of food-borne pathogens isolated from fruit, vegetables and sprouts retailed in the Czech Republic. Food Microbiology. 63: 147-152. [DOI:10.1016/j.fm.2016.11.012] [PMID]
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