Volume 7, Issue 4 (December 2020)
J. Food Qual. Hazards Control 2020, 7(4): 181-187 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Eslami G, Peletto S, Vakili M, Kargar S. Effect of Electron Beam Irradiation on Viability of Sarcocystis spp. in Beef. J. Food Qual. Hazards Control 2020; 7 (4) :181-187
URL: http://jfqhc.ssu.ac.ir/article-1-808-en.html
URL: http://jfqhc.ssu.ac.ir/article-1-808-en.html
Department of Food Hygiene and Safety, School of Public Health, Shahid Sadoughi University of Medical Sciences, Yazd, Iran , samanekargar3@gmail.com
Abstract: (1338 Views)
Background: Sarcocystosis is one of the most distributed parasitic diseases over the world, caused by Sarcocystis spp. In this study, we assessed the effect of electron beam irradiation on the viability of Sarcocystis spp. in beef.
Methods: Experimental beef groups were irradiated by four different electron beam doses of 1, 2, 3, and 4 kGy, at intervals of 0 and 24 h after irradiation, then the samples were transferred inside a sterile microtube containing RNAlater solution and stored at -20 °C till next steps. RNA extractions and cDNA synthesis were done using the related kit in order to detect the presence of the 18S ribosomal RNA region. Relative quantification was carried out using SYBR Green Real time Polymerase Chain Reaction. The statistical analysis was done using SPSS 16.0 by Tukey’s and Kruskal-Wallis tests.
Results: Irradiation at zero time was not effective on viability of Sarcocystis, but at 24 h, irradiation doses of 3 (p=0.003) and 4 kGy (p=0.008) caused a significant reduction in Sarcocystis viability. Irradiation doses of 1 and 2 kGy had no significant (p>0.05) effect on Sarcocystis viability reduction. Also, no significant differences (p>0.05) were observed between irradiation doses of 3 and 4 kGy.
Conclusion: Electron beam radiation at dose of 3 kGy was effective as the optimal dose for the elimination of Sarcocystis spp. in beef.
DOI: 10.18502/jfqhc.7.4.4846
Methods: Experimental beef groups were irradiated by four different electron beam doses of 1, 2, 3, and 4 kGy, at intervals of 0 and 24 h after irradiation, then the samples were transferred inside a sterile microtube containing RNAlater solution and stored at -20 °C till next steps. RNA extractions and cDNA synthesis were done using the related kit in order to detect the presence of the 18S ribosomal RNA region. Relative quantification was carried out using SYBR Green Real time Polymerase Chain Reaction. The statistical analysis was done using SPSS 16.0 by Tukey’s and Kruskal-Wallis tests.
Results: Irradiation at zero time was not effective on viability of Sarcocystis, but at 24 h, irradiation doses of 3 (p=0.003) and 4 kGy (p=0.008) caused a significant reduction in Sarcocystis viability. Irradiation doses of 1 and 2 kGy had no significant (p>0.05) effect on Sarcocystis viability reduction. Also, no significant differences (p>0.05) were observed between irradiation doses of 3 and 4 kGy.
Conclusion: Electron beam radiation at dose of 3 kGy was effective as the optimal dose for the elimination of Sarcocystis spp. in beef.
DOI: 10.18502/jfqhc.7.4.4846
Keywords: Radiation, Sarcocystis, Red Meat, Survival, Microbial Viability, Real-Time Polymerase Chain Reaction
Type of Study: Original article |
Subject:
Special
Received: 19/03/02 | Accepted: 19/12/08 | Published: 20/12/24
Received: 19/03/02 | Accepted: 19/12/08 | Published: 20/12/24
References
1. Al-Bachir M., Zeinou R. (2009). Effect of gamma irradiation on microbial load and quality characteristics of minced camel meat. Meat Science. 82: 119-124. [DOI: 10.1016/j.meatsci. 2008.12.012] [DOI:10.1016/j.meatsci.2008.12.012] [PMID]
2. Al-Farisi M., Abuagla A., Mohamed E., Gohs U. (2013). The effect of electron beam on dates infestation. Food Control. 33: 157-161. [DOI: 10.1016/j.foodcont.2013.02.029] [DOI:10.1016/j.foodcont.2013.02.029]
3. Collins M.V., Flick G.J., Smith S.A., Fayer R., Croonenberghs R., O'Keefe S., Lindsay D.S. (2005). The effect of high-pressure processing on infectivity of Cryptosporidium parvum oocysts recovered from experimentally exposed Eastern oysters (Crassostrea virginica). The Journal of Eukaryotic Microbiology. 52: 500-504. [DOI: 10.1111/j.1550-7408.2005.00059.x] [DOI:10.1111/j.1550-7408.2005.00059.x] [PMID]
4. Dubey J.P., Calero-Bernal R., Rosenthal B.M., Speer C.A., Fayer R. (2016). Sarcocystosis of animals and man. 2nd edition. CRC Press, Boca Raton. [DOI:10.1201/b19184]
5. Dubey J.P., Lindsay D.S. (2006). Neosporosis, toxoplasmosis, and sarcocystosis in ruminants. Veterinary Clinics of North America: Food Animal Practice. 36: 645-671. [DOI: 10.1016/j.cvfa. 2006.08.001] [DOI:10.1016/j.cvfa.2006.08.001] [PMID]
6. Ehlermann D.A. (2016). Particular applications of food irradiation: meat, fish and others. Radiation Physics and Chemistry. 129: 53-57. [DOI: 10.1016/j.radphyschem.2016.07.027] [DOI:10.1016/j.radphyschem.2016.07.027]
7. Farkas J. (1998). Irradiation as a method for decontaminating food: a review. International Journal of Food Microbiology. 44: 189-204. [DOI: 10.1016/S0168-1605(98)00132-9] [DOI:10.1016/S0168-1605(98)00132-9]
8. Farkas J., Mohácsi-Farkas C. (2011). History and future of food irradiation. Trends in Food Science and Technology. 22: 121-126. [DOI: 10.1016/j.tifs.2010.04.002] [DOI:10.1016/j.tifs.2010.04.002]
9. Fayer R., Esposito D.H., Dubey J.P. (2015). Human infections with Sarcocystis species. Clinical Microbiology Reviews. 28: 295-311. [DOI: 10.1128/CMR.00113-14] [DOI:10.1128/CMR.00113-14] [PMID] [PMCID]
10. Franssen F., Gerard C., Cozma-Petrut A., Vieira-Pinto M., Jambrak A.R., Rowan N., Paulsen P., Rozycki M., Tysnes K., Rodriguez-Lazaro D., Robertson L. (2019). Inactivation of parasite transmission stages: efficacy of treatments on food of animal origin. Trends in Food Science and Technology. 83: 114-128. [DOI: 10.1016/j.tifs.2018.11.009] [DOI:10.1016/j.tifs.2018.11.009]
11. Górska-Warsewicz H., Laskowski W., Kulykovets O., Kudlińska-Chylak A., Czeczotko M., Rejman K. (2018). Food products as sources of protein and amino acids-the case of Poland. Nutrients. 10: 1977. [DOI: 10.3390/nu10121977] [DOI:10.3390/nu10121977] [PMID] [PMCID]
12. Hajimohammadi B., Eslami G., Oryan A., Zohourtabar A., Pourmirzaei Tafti H., Moghaddam Ahmadi M. (2014). Molecular identification of Sarcocystis hominis in native cattle of central Iran: a case report. Tropical Biomedicine. 31:183-186.
13. Hallman G.J. (2013). Control of stored product pests by ionizing radiation. Journal of Stored Products Research. 52: 36-41. [DOI: 10.1016/j.jspr.2012.10.001] [DOI:10.1016/j.jspr.2012.10.001]
14. International Atomic Energy Agency (IAEA). (2015). Manual of good practice in food irradiation: sanitary, phytosanitary and other applications. Technical reports series No. 481. International Atomic Energy Agency.
15. Javanmard M., Rokni N., Bokaie S., Shahhosseini G. (2006). Effects of gamma irradiation and frozen storage on microbial, chemical and sensory quality of chicken meat in Iran. Food Control. 17: 469-473. [DOI: 10.1016/j.foodcont.2005.02.008] [DOI:10.1016/j.foodcont.2005.02.008]
16. Lung H.M., Cheng Y.C., Chang Y.H., Huang H.W., Yang B.B., Wang C.Y. (2015). Microbial decontamination of food by electron beam irradiation. Trends in Food Science and Technology. 44: 66-78. [DOI: 10.1016/j.tifs.2015.03.005] [DOI:10.1016/j.tifs.2015.03.005]
17. McFadden E., Costa-Ramos A.L., Bradley D., Vrain O., McEvoy B., Rowan N.J. (2016). Comparative studies on the novel sterilisation of Irish retailed infant milk formula using electron beam and pulsed light treatments. International Journal of Science, Environment and Technology. 5: 4375-4377.
18. Moghadam Ahmadi M., Hajimohammadi B., Eslami G., Oryan A., Yasini Ardakani S.A., Zohourtabar A., Zare S. (2015). First identification of Sarcocystis hominis in Iranian traditional hamburger. Journal of Parasitic Diseases. 39: 770-772. [DOI: 10.1007/s12639-014-0425-7] [DOI:10.1007/s12639-014-0425-7] [PMID] [PMCID]
19. Munir M.T., Federighi M. (2020). Control of foodborne biological hazards by ionizing radiations. Foods. 9: 878. [DOI: 10.3390/foods9070878] [DOI:10.3390/foods9070878] [PMID] [PMCID]
20. Murray K.A., Kennedy J.E., Barron V., McEvoy B., Vrain O., Ryan D., Cowman R., Higginbotham C.L. (2015). Effects of electron beam irradiation on the property behaviour of poly (ether-block-amide) blended with various stabilisers. Radiation Physics and Chemistry. 110: 24-37. [DOI: 10.1016/ j.radphyschem.2015.01.009] [DOI:10.1016/j.radphyschem.2015.01.009]
21. Murrell K.D. (1995). Foodborne parasites. International Journal of Environmental Health Research. 5: 63-85. [DOI: 10.1080/ 09603129509356833] [DOI:10.1080/09603129509356833]
22. Ooi H.K., Ishii K., Inohara J., Kamiya M. (1993). Effect of irradiation on the viability of Angiostrongylus cantonensis and A. costaricensis infective larvae. Journal of Helminthology. 67: 238-242. [DOI: 10.1017/S0022149X00013183] [DOI:10.1017/S0022149X00013183] [PMID]
23. Pohle S., Ernst R., MacKenzie C., Spicher M., Romig T., Hemphill A., Gripp S. (2011). Echinococcus multilocularis: the impact of ionizing radiation on metacestodes. Experimental Parasitology. 127: 127-134. [DOI: 10.1016/j.exppara.2010.07.006] [DOI:10.1016/j.exppara.2010.07.006] [PMID]
24. Rahman M.S. (2007). Handbook of food preservation. CRC press, UK. [DOI:10.1201/9781420017373]
25. Yu J.R., Park W.Y. (2003). The effect of γ-irradiation on the viability of Cryptosporidium parvum. Journal of Parasitology. 89: 639-642. [DOI: 10.1645/0022-3395(2003)089[0639: TEOIOT]2.0.CO;2] [DOI:10.1645/0022-3395(2003)089[0639:TEOIOT]2.0.CO;2]
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
