Journal of food quality and hazards control

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Molecular Epidemiology and Antimicrobial Resistance of Salmonella spp. Isolated from Resident Patients in Mazandaran Province, Northern Iran

M.m. Soltan Dallal , M. Khalilian , H. Masoumi Asl , R. Bakhtiari , A. Davoodabadi , M.k. Sharifi Yazdi , P. Torabi Bonabi ,
Volume 3, Issue 4 (December 2016)

Abstract

Background: Salmonella is one of the causes of food-borne illnesses worldwide resulting in a wide range of human diseases. The aim of this research was to study molecular epidemiology and antimicrobial resistance of Salmonella spp. isolated from resident patients in Mazandaran province, northern Iran.

Methods: Totally, 51 stool samples were collected from patients in 20 outbreaks recorded in Health Care Center of Mazandaran province, North of Iran, during October 2013 to September 2014. Cultivation and biochemical methods were used for differentiation of Salmonella. Identified Salmonella isolates were confirmed with 16S rRNA PCR method and sequencing. Antibiotic susceptibility test was carried out for each isolate.

Results: Four of 51 samples (7.84%) were contaminated with S. enteritidis. Molecular analysis and sequencing verified the isolates. The delineation level for each isolate discrimination in subsequent analysis was set at 94% for the primer. All of the isolates were resistant to tetracycline, nalidixic acid, and trimethoprim-sulfamethoxazole. No resistance was found to cefotaxime, chloramphenicol, ciprofloxacin, amoxicillin, and ceftazidime.

Conclusion: S. enteritidis was the most frequent serovar among salmonellosis outbreaks in the Mazandaran province, Iran. Also, isolated S. enteritidis showed various rates of susceptibility for different antibiotics.

Investigation of Enterotoxin-Producing Genes (sea, seb, sec, and sed) in Staphylococcus aureus Isolated from Raw Traditionally and Pasteurized Milk Supplied in Tehran, Iran

Z. Rajabi , A. Monadi Sefidan , M. Zarebavani , S. Sharifi Yazdi , S. Sharifi Yazdi , P. Torabi Bonab , S.z. Mirbagheri , M.m. Soltan- Dallal ,
Volume 10, Issue 4 (December 2023)

Abstract

Background: The issue of milk quality appears to be vital due to its nutritional value and since raw milk can be regarded as an appropriate environment for the growth of several pathogens by producing an enterotoxin. The aim of present study is to investigate enterotoxin-producing genes (sea, seb, sec, and sed) from Staphylococcus aureus isolated from raw traditional and pasteurized milk in Tehran, Iran.
Methods: One hundred and fifty samples of raw traditional milk supplied in five districts of Tehran were collected and simultaneously15 pasteurized milk samples from various brands were prepared and examined phenotypically and bio-chemically for the existence of S. aureus. The presence of sea, seb, sec, sed genes was assessed by Polymerase Chain Reaction and ultimately the antibiotic residue was measured with a commercial kit.
Results: In this study, 32 (21.3%) samples of raw traditional milk and one sample (6.7%) of pasteurized milk samples were infected with S. aureus. The frequency of presence of sea, seb, sec, and sed genes regarded to be: 9 (28.12%), 14 (43.75%), 6 (18.75%), 2 (6.25%), respectively however sec gene failed to identify. Basically 38 (33.25%) of raw traditional milk samples as well as 5 (33.33%) of pasteurized milk included antibiotic residue.
Conclusion: The high prevalence of S. aureus comprising enterotoxin genes in raw traditional milk is considered as a severe warning to the community and highlights the need for a high quality product.

DOI: 10.18502/jfqhc.10.4.14180

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