Journal of food quality and hazards control

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Prevalence of Multidrug Resistant Shiga Toxin-Producing Escherichia coli in Cattle Meat and Its Contact Surfaces

W.s. Darwish , A.s. Atia , W.r. El-Ghareeb , A.e. Elhelaly ,
Volume 5, Issue 4 (12-2018)

Abstract

Background: Shiga toxin-producing Escherichia coli (STEC) are group of E. coli causing bloody diarrhea. The goal of this survey was to determine the prevalence of multidrug resistant shiga toxin-producing E. coli in cattle meat and its contact surfaces.
Methods: Swab samples (n=120) were randomly collected from meat and contact surface of butchery shops in Sharkia province, Egypt. Prevalence of E. coli was examined using culture, biochemical, and serological methods. Identification of shiga toxin-encoding genes (stx1 and stx2) in the E. coli serotypes was done using multiplex polymerase chain reaction. Screening of multidrug resistance profile was done using the disk-diffusion method. Data were analyzed using JMP statistical package, SAS Institute Inc., Cary, NC.
Results: The prevalence rates of E. coli in the chuck, round, masseter muscles, cutting-boards, walls, and floors were 20, 10, 30, 50, 40, and 60%, respectively. Among the isolates, E. coli O111:H4 and E. coli O26:H11 harbored the two mentioned genes. E. coli O86 and E. coli O114:H21 harbored only stx1; while E. coli O55:H7 encoded only stx2. Just E. coli O124 had no express of stx1 and stx2. The isolated E. coli serovars showed a multidrug resistance profile.
Conclusion: Considering the results of this study, strict hygienic procedures should be followed to avoid or reduce carcass cross-contamination. In addition, proper handling and efficient cooking of meat are highly recommended by consumers to reduce the risk of human exposure to STEC.

DOI: 10.29252/jfqhc.5.4.6

Multidrug Resistance and Virulence Factors of Enterococci Isolated from Milk and Some Dairy Desserts

O.a. Sadek , A.m. Koriem ,
Volume 9, Issue 4 (12-2022)

Abstract

Background: Enterococci spp. bacteria especially Enterococcus faecalis and E. faecium have the ability to acquire antibiotic-resistance pattern and causing life-threatening hospital-acquired infections. So, the aim of this study was to count and isolate of E. faecalis and E. faecium from milk and dairy desserts consumed in Assiut city, Egypt.
Methods: A total of 100 raw milk, ice cream, mehallabia, and milk rice samples were collected from dairies shop in Assiut city, Egypt and were bacteriologically examined for the presence and count of Enterococcus spp. Then, identification of enterococci isolates by conventional and Polymerase Chain Reaction (PCR) methods, performance of antibiotic sensitivity assay, and some virulence genes in the Multi Drug Resistant (MDR) isolates were identified.
Results: The prevalence of counted Enterococcus spp. in raw milk, ice cream, mehallabia, and milk rice samples were 76, 44, 20, and 32%, respectively. The prevalence of E. faecalis in raw milk, ice cream, mehallabia, and milk rice samples were 64, 0, 0, and 8%, while for E. faecium were 12, 44, 20, and 24%, respectively. E. faecalis isolates were resistant to vancomycin, ciprofloxacin, gentamicin, erythromycin, and tetracycline with the rate of 72.2, 88.9, 88.9, 94.4, and 77.8%, respectively, while for the resistance rates of E. faecium were 16, 40, 16, 84, and 20%, respectively. E. faecalis and E. faecium were MDR in rate of 88.9 and 32%, respectively.
Conclusion: This study revealed that milk, ice cream, mehallabia, and milk rice could be a source of enterococci to consumers in Assiut, Egypt. Moreover, E. faecalis had higher MDR and Resistant Index (RI) than E. faecium.

DOI: 10.18502/jfqhc.9.4.11376

Parasitic Contamination in Five Leafy Vegetables Collected from Open Marketplaces in Giza, Egypt

N.m. El-Sayed , S.s. Gawdat , H.s. El-Kholy , A. Elmosalamy ,
Volume 10, Issue 1 (3-2023)

Abstract

Background: Leafy vegetables could be contaminated with parasitic infective stages, making them possible sources of human parasitic infections. This study aimed to assess parasites contamination level among five different leafy vegetables collected from open marketplaces in Giza, Egypt.
Methods: Hundred vegetable samples were collected from dill, parsley, coriander, rocca, and mint (20 of each). Samples were processed using sedimentation and concentration methods and the sediments were examined as wet smears either unstained or stained with Lugol's iodine. Also, modified trichrome and Ziehl-Neelsen stains were used to detect Microsporidia spores and coccidian oocysts. Statistical software SPSS version 20 was used for data analysis.
Results: A total of 86% of the examined samples were found contaminated with parasite's eggs, cysts, oocysts, and larvae. Coriander was the most contaminated vegetable with contamination rate of 95% while dill was the least contaminated (80%). Also, protozoan parasites contamination rate (77.1%) was higher than helminths parasites contamination rate (22.9%). Entamoeba histolytica/dispar was the most prevalent parasite (40.6%) followed by Blastocystis hominis (39.5%), Ascaris lumbricoides (18.6%), Cyclospora spp. (15.11%), Giardia lamblia (11.6%), E. coli (9.3%); while Cryptosporidium spp., Iodamoeba bütschlii, Trichuris trichiura, Enterobius vermicularis, Fasciola, and hookworms’ eggs were the least prevalent parasites (1.1%).
Conclusion: Leafy vegetables in Giza, Egypt had an extremely high parasite contamination rate. So, consuming such vegetables without proper washing facilitates transmission of parasitic infections which could pose a major health risk to Egyptians.

DOI: 10.18502/jfqhc.10.1.11984

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