Journal of Food Quality and Hazards Control

en Hydroxymethylfurfural Content and Sugar Profile of Honey Available in Bangladesh Using Validated HPLC-PDA and HPLC-RID تخصصي Special Original article Original article <div style="text-align: justify;">Background: Honey has a lot of reputation because of its supposed medicinal properties. In this study, Hydroxymethylfurfural (HMF), sugars, and Fructose/Glucose ratio of honey in Bangladesh were assessed for adulteration and authenticity evaluation. Methods: Seventy honey samples collected from different districts of Bangladesh were analyzed by High Performance Liquid Chromatography (HPLC) for HMF content and sugar profile. The samples were prepared by using Carrez I and Carrez II prior to injecting into HPLC. The samples were then filtered through syringe filter and taken in 1.5 ml vial for injecting into the HPLC system. Results: HMF values were ranging from 1.41 mg/kg to 2,063.90 mg/kg. The Limit of Detection (LOD) and Limit of Quantification (LOQ) was found 0.10 mg/kg and 0.33 mg/kg with R2=0.9994. The average values of fructose, glucose, and sucrose were in the range of 14.75-52.44%, 8.19-42.63%, and 0.10-21.12%, respectively. From validation parameters, LOD values for fructose, glucose, and sucrose were 0.003, 0.008, and 0.004%, respectively; and LOQ values were 0.01, 0.028, and 0.015%, respectively with an excellent linearity with R2 for fructose=1.0, glucose=0.9999, and sucrose=1.0. Conclusion: Some samples had higher HMF content which may be due to the storage time was increased and improper processing with high temperature or adulteration by High Fructose Corn Syrup (HFCS), sugar cane syrup, rice syrups or rice molasses. The sugar profiles showed that the most of honey samples were nectar honeys. DOI: 10.18502/jfqhc.9.3.11154</div> Honey, 5-Hydroxymethylfurfural, Chromatography, High Pressure Liquid, Fraud, Sucrose, Bangladesh 160 168 http://jfqhc.ssu.ac.ir/browse.php?a_code=A-10-1488-2&slc_lang=en&sid=1 S. Das sumanbcsir@gmail.com 0000-0002-2743-8323 Yes Bangladesh Council of Scientific and Industrial Research (BCSIR) Chattogram Laboratories, Chattogram-4,220, Bangladesh M.N. Uddin No Department of Chemistry, University of Chittagong, Chittagong-4,331, Bangladesh M.S. Haque No Department of Chemistry, University of Chittagong, Chittagong-4,331, Bangladesh D. Chakraborty No Bangladesh Council of Scientific and Industrial Research (BCSIR) Chattogram Laboratories, Chattogram-4,220, Bangladesh M. Mostafa No Bangladesh Council of Scientific and Industrial Research (BCSIR) Chattogram Laboratories, Chattogram-4,220, Bangladesh A. Hasnaine No Department of Chemistry, University of Chittagong, Chittagong-4,331, Bangladesh S.K. Das No Bangladesh Council of Scientific and Industrial Research (BCSIR) Chattogram Laboratories, Chattogram-4,220, Bangladesh M. Uddin No Department of Chemistry, University of Chittagong, Chittagong-4,331, Bangladesh