Journal of Food Quality and Hazards Control

en Investigation of Enterotoxin-Producing Genes (sea, seb, sec, and sed) in Staphylococcus aureus Isolated from Raw Traditionally and Pasteurized Milk Supplied in Tehran, Iran تخصصي Special Original article Original article <div style="text-align: justify;">Background: The issue of milk quality appears to be vital due to its nutritional value and since raw milk can be regarded as an appropriate environment for the growth of several pathogens by producing an enterotoxin. The aim of present study is to investigate enterotoxin-producing genes (sea, seb, sec, and sed) from Staphylococcus aureus isolated from raw traditional and pasteurized milk in Tehran, Iran. Methods: One hundred and fifty samples of raw traditional milk supplied in five districts of Tehran were collected and simultaneously15 pasteurized milk samples from various brands were prepared and examined phenotypically and bio-chemically for the existence of S. aureus. The presence of sea, seb, sec, sed genes was assessed by Polymerase Chain Reaction and ultimately the antibiotic residue was measured with a commercial kit. Results: In this study, 32 (21.3%) samples of raw traditional milk and one sample (6.7%) of pasteurized milk samples were infected with S. aureus. The frequency of presence of sea, seb, sec, and sed genes regarded to be: 9 (28.12%), 14 (43.75%), 6 (18.75%), 2 (6.25%), respectively however sec gene failed to identify. Basically 38 (33.25%) of raw traditional milk samples as well as 5 (33.33%) of pasteurized milk included antibiotic residue. Conclusion: The high prevalence of S. aureus comprising enterotoxin genes in raw traditional milk is considered as a severe warning to the community and highlights the need for a high quality product. DOI: 10.18502/jfqhc.10.4.14180</div> Staphylococcus aureus, Milk, Enterotoxins, Polymerase Chain Reaction, Iran 221 225 http://jfqhc.ssu.ac.ir/browse.php?a_code=A-10-152-15&slc_lang=en&sid=1 Z. Rajabi 0000-0003-4970-3068 No Zoonoses Research Center, Tehran University of Medical Sciences, Iran, Food Microbiology Research Center, Tehran University of Medical Sciences, Iran A. Monadi Sefidan No Department of Laboratory Medical Sciences, School of Paramedicine, Tehran University of Medical Sciences, Iran M. Zarebavani No Department of Laboratory Medical Sciences, School of Paramedicine, Tehran University of Medical Sciences, Iran S. Sharifi Yazdi No Faculty of Medicine, Tehran University of Medical Sciences, Iran S. Sharifi Yazdi No Faculty of Medicine, Tehran University of Medical Sciences, Iran P. Torabi Bonab No Food Microbiology Research Center, Tehran University of Medical Sciences, Iran S.Z. Mirbagheri No Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Iran. M.M. Soltan- Dallal msoltandallal@gmail.com 0000-0002-3421-3974 Yes Food Microbiology Research Center, Tehran University of Medical Sciences, Iran, Devision of Food Microbiology, Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Iran