en تخصصي Special Original article Original article <div align="justify"><font face="times new roman,times,serif" size="2"><b>Background:</b> <i>Pseudomonas </i>spp. are of important food spoiling bacteria. They present around the world and are isolated from diverse sources. The main objective of this survey was to detect <i>Pseudomonas</i> spp. in fresh chicken and fish obtained from markets of Assiut city, Egypt.&nbsp;<br> <b>Methods:</b> In this cross-sectional survey, 50 samples of fresh raw chicken and 50 samples of fresh raw fish meat were randomly collected from Assiut city markets, Egypt. Then, in laboratory, cultural and PCR techniques were carried out to detect <i> Pseudomonas</i> spp. in the samples.<br> <b>Results:</b> <i>Pseudomonas</i> spp. were isolated from 80% of the examined chicken samples and found in all the examined fish samples. All the isolates were confirmed as <i>Pseudomonas</i> spp. using <i>16S rRNA</i>-PCR. The average number of <i>Pseudomonas</i> spp. in chicken flesh and <i>Tilapia nilotica</i> were 2.6&times;10⁴ and 2.8&times;10³ CFU/g, respectively while this rate was 1.4&times;10⁵ CFU/g for African catfish (<i>Clarias gariepinus</i>).<br> <b>Conclusions:</b> This study indicated contamination of fresh raw fish and chicken samples with<i> Pseudomonas</i> spp. which may be attributed to cross contamination. This contamination predicts inadequate shelf life of the examined samples especially African catfish. </font></div> Chickens, Fish Products, Pseudomonas, Food Quality 86 89 http://jfqhc.ssu.ac.ir/browse.php?a_code=A-10-1-108&slc_lang=en&sid=1 D.M. Abd El-Aziz doaassiut@yahoo.com Yes