en تخصصي Special Original article Original article <p style="text-align: justify"><span style="font-family:times new roman"><strong>Background:</strong> Sarcocystosis in South American camelids (SAC) is an important parasitic disease which results in economical loss due to carcass condemnation. Meat products from camelids are significant source of animal protein in several American countries.<em> Sarcocystis</em> spp. producing macroscopical cysts in these animals have been nominated as <em>S. aucheniae</em>, <em>S. tilopodi</em>, and <em>S. guanicoecanis</em>. The aim of the present study was to characterize morphologically and molecularly <em>Sarcocystis</em> spp. producing macroscopical cysts in guanacos (<em>Lama guanicoe</em>).</span></p> <p style="text-align: justify"><span style="font-family:times new roman"><strong>Methods:</strong> Samples of macroscopical cysts were dissected from guanaco muscles slaughtered in Santa Cruz province, Argentina. The samples were processed by transmission electron microscopy, polymerase chain reaction (PCR), cloning and sequencing studies.</span></p> <p style="text-align: justify"><span style="font-family:times new roman"><strong>Results:</strong> Cyst wall villar protrusions were irregular with a &ldquo;tree&rdquo; or &ldquo;cauliflower-like&rdquo; shape which measured 3-4.5 &micro;m height by 2.5-3.5 &micro;m width. A consensus <em>18S rRNA</em> full gene sequence of 1875 bp was obtained and showed the highest identity (</span>&ge;<span style="font-family:times new roman"> 99%) with sequences of <em>S. aucheniae</em> reported in GenBank. The ultra structure of the cyst wall from macroscopical cysts and <em>18S rRNA</em> gene obtained from guanacos were identical and consistent with the descriptions of <em>S. aucheniae</em>.</span></p> <p style="text-align: justify"><span style="font-family:times new roman"><strong>Conclusion:</strong> This study confirms that guanacos are infected by the same <em>Sarcocystis </em>spp. affecting other SAC, named <em>S. aucheniae</em>, and reinforce the suggestion that nomination of new species should contain morphological and molecular data to reach validity. In future, this data could be useful to obtain proper identifications in post-mortem inspection and helpful to improve meat safety.</span></p> Sarcocystis, Polymerase Chain Reaction, Sequence Analysis, DNA, Microscopy, Electron 118 121 http://jfqhc.ssu.ac.ir/browse.php?a_code=A-10-1-115&slc_lang=en&sid=1 C. Regensburger No Unidad Académica Caleta Olivia, Universidad Nacional de la Patagonia Austral, Santa Cruz, Argentina M.L. Gos No Laboratorio de Inmunoparasitología, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, La Plata J. Ctibor No Unidad Académica Caleta Olivia, Universidad Nacional de la Patagonia Austral, Santa Cruz, Argentina G. Moré gastonmore@fcv.unlp.edu.ar Yes Laboratorio de Inmunoparasitología, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, La Plata