en تخصصي Special Original article Original article <p style="text-align: justify;"><span style="font-family:times new roman;"><strong>Background:</strong> Deoxynivalenol (DON) is a mycotoxin produced mainly by <em>Fusarium graminearum</em> in grains such as wheat and maize. The aim of this study was to evaluate the inhibitory effects of citrus flavanones including, naringin (NAR), hesperidin (HES), and neohesperidin (NEO) on deoxynivalenol production using Response Surface Methodology (RSM).</span></p> <p style="text-align: justify;"><span style="font-family:times new roman;"><strong>Methods:</strong> The studied flavanones were extracted from residues of citric industries and assayed in rice media inoculated with <em>F. graminearum. </em>After Gas Chromatography (GC) analysis, RSM was applied to find the optimal flavanones concentrations that would lead to total inhibition of DON production. The four levels studied were 0, 0.11, 0.21, and 0.42 mmol/kg rice in dry basis, for each flavanone. Statistical analysis was performed using the statgraphics centurion XV package, version 15.2.6 (StatPoint Technologies, USA). Experimental design consisted in ten factorial points was evaluated in triplicate and used in the model.</span></p> <p style="text-align: justify;"><span style="font-family:times new roman;"><strong>Results:</strong> All flavanones and their mixtures significantly decreased the accumulation of DON in rice media respect to control (<em>p</em><0.05). NEO, when applied alone, was the only flavanone that could reach 100% inhibition of DON accumulation in all concentrations tested, followed by HES and NAR that could only reach total inhibition of DON at 0.21 and 0.42 mmol/kg, respectively. Only the mixtures NAR-HES 0.11-0.11 mmol/kg and NEO-HES 0.11-0.11mmol/kg could not completely inhibit DON accumulation in comparison with other mixtures. The obtained optimal combinations were HES-NAR 0.050-0.236, HES-NEO 0.046-0.217, and NAR-NEO 0.034-0.193 mmol/kg rice in dry basis.</span></p> <p style="text-align: justify;"><span style="font-family:times new roman;"><strong>Conclusion:</strong> Using RSM, the citric flavanones studied in this work, was proved to be effective for total inhibition of DON accumulation.</span></p> Flavanones, Fusarium, Deoxynivalenol, Prevention and Control 41 47 http://jfqhc.ssu.ac.ir/browse.php?a_code=A-10-235-11&slc_lang=en&sid=1 M.P. Salas No Organic Chemistry and Industry Department, Faculty of Natural Sciences, University of Buenos Aires, Intendente Güiraldes 2160, C1428EGA, Buenos Aires, Argentina P.S. Pok No Organic Chemistry and Industry Department, Faculty of Natural Sciences, University of Buenos Aires, Intendente Güiraldes 2160, C1428EGA, Buenos Aires, Argentina S.L. Resnik No Organic Chemistry and Industry Department, Faculty of Natural Sciences, University of Buenos Aires, Intendente Güiraldes 2160, C1428EGA, Buenos Aires, Argentina A.M. Pacin benedicta@ictbdelacruz.org.ar Yes Scientific Research Foundation Teresa Benedicta de la Cruz, Dorronzoro 141, B6700, Luján, Buenos Aires, Argentina M.S. Munitz No Food Science Faculty, Entre Ríos National University, Monsenor Tavella 1450, Concordia, Argentina