en تخصصي Special Case report Case report <p align="justify"><font face="times new roman,times,serif" size="2"><strong>Introduction: </strong>The main agents of sarcocystosis in cattle as an intermediate host include <i>S. cruzi</i>, <i>S. hominis</i> and <i>S. hirsuta</i>. A sensitive and specific tool such as molecular-based techniques would be necessary to identify the species. </font></p> <p align="justify"><font face="times new roman,times,serif" size="2"><strong>Case report: </strong>After collection of beef sample from Yazd slaughterhouse, DNA extraction was done with salting out method. The <i>18SrRNA</i> gene as a specific target gene was used for molecular detection of <i>Sarcocystis</i> spp, then Restriction Fragment Length Polymorphysm (RFLP) analysis identified the speciesusing <i>Rsa </i>and <i>Bfa.</i> Results showed that our designed molecular method could identify <i>S. hirsuta</i> in beef sample. </font></p> <p align="justify"><font face="times new roman,times,serif" size="2"><strong>Conclusion: </strong>Based on our knowledge, this study indicates the first report of molecular identification of <i>S. hirsuta</i> in Iran. </font></p> Sarcocystis, Molecular diagnostic techniques, Meat, Iran 32 34 http://jfqhc.ssu.ac.ir/browse.php?a_code=A-10-1-18&slc_lang=en&sid=1 G. Eslami No Department of Parasitology and Mycology, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran A. Zohourtabar azohourtabar2000@gmail.com Yes Department of Food Hygiene and Safety, Faculty of Health, Shahid Sadoughi University of Medical Sciences, Yazd, Iran S.R. Mehrizi No Official Meat Inspector at Yazd Industrial Slaughterhouse, Yazd, Iran