en تخصصي Special Original article Original article <div style="text-align: justify;"><span style="font-family:times new roman;"><span style="font-size:14px;"><strong>Background: </strong>In food industry, carotenoids are used as food colorants conferring yellow to red color. This research was designed to study on lipid and &beta;-carotene production by <em>Rhodosporidium diobovatum</em> cultured with different Carbon to Nitrogen (C/N) ratios.<br> <strong>Methods:</strong> <em>R. diobovatum</em> was cultured in a medium containing 40 g/l glucose (as the carbon source) and different C/N ratios (20, 50, and 80), which were established by adding different amounts of (NH₄)₂SO₄ (3.78, 1.51, and 0.94 g/l) as the source of nitrogen. High performance liquid chromatography, gas chromatography, and microplate reader were used to determine the glucose concentration, lipid production, and &beta;-carotene concentration, respectively. Data were analyzed using IBM SPSS statistics (v. 24).<br> <strong>Results:</strong> Cultures with a C/N ratio of 50 produced the greatest amount of lipids during 120 h pi. However, lipid synthesis in the first 48 h pi was very low for all three C/N ratios. Analyses of the lipid composition revealed that oleic acid and linoleic acid were the dominant (60%) fatty acids. Cultures with a C/N ratio of 50 also produced the greatest amount of &beta;-carotene.<br> <strong>Conclusion:</strong> <em>R. diobovatum</em> in the C/N of 50 culture medium resulted in greater concentrations of lipid and &beta;-carotene. Defining the optimum C/N ratio will enable development of optimized bioprocess engineering parameters for scale-up production of lipid and &beta;-carotene in food industries by this yeast species.</span></span></div> <br> <span style="font-family:times new roman;"><span style="font-size:14px;"><strong>DOI:</strong> 10.18502/jfqhc.6.2.956</span></span> Yeasts, Lipids, Carotenoids, Food Industry 58 65 http://jfqhc.ssu.ac.ir/browse.php?a_code=A-10-573-4&slc_lang=en&sid=1 M. Mirzaie mmirzaei29@gmail.com 0000-0001-5796-559X Yes Department of Biosystems Engineering, University of Manitoba, Winnipeg, MB R3T 5V6, Canada S.S. Saei-Dehkordi No Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, University of Shahrekord, Shahrekord, Iran D.B. Levin No Department of Biosystems Engineering, University of Manitoba, Winnipeg, MB R3T 5V6, Canada