Journal of Food Quality and Hazards Control مجله کیفیت و کنترل مخاطرات مواد غذایی J. Food Qual. Hazards Control Medical Sciences http://jfqhc.ssu.ac.ir 1 admin 2345-685X 2345-6825 8 10.29252/jfqhc 14 8888 13 en jalali 1400 6 1 gregorian 2021 9 1 8 3 online 1 fulltext
en Investigation of TEM and SHV Beta-Lactamase Genes in Escherichia coli Isolated from Strawberry Samples in Sanandaj, Iran تخصصي Special Original article Original article <div style="text-align: justify;"><span style="font-size:14px;"><span style="font-family:Times New Roman;"><strong>Background: </strong>When animal manures are used, food products may include pathogenic bacteria, especially <em>Escherichia coli</em>. The major aim of the current study was to investigate TEM (<em>bla</em><sub>TEM</sub>) and SHV beta-lactamase (<em>bla</em><sub>SHV</sub>) genes in <em>E. coli</em> isolated from strawberry samples in Sanandaj, Iran.<br> <strong>Methods:</strong> In this study, 150 strawberry samples were collected from farms (traditional), greenhouses, and packages in Sanandaj, Iran. <em>E. coli</em> contamination was done using routine culture methods. Then, isolates were investigated for Extended-Spectrum Beta-Lactamase (ESBL) production and <em>bla</em><sub>TEM</sub> or <em>bla</em><sub>SHV</sub> genes using phenotypic and genotypic methods, respectively.<br> <strong>Results:</strong> The most susceptibility and resistance of <em>E. coli</em> to antibiotics were related to chloramphenicol and trimethoprim/sulfamethoxazole, respectively. Out of 21 isolates of <em>E. coli</em>, eight were resistant to ceftazidime and cefotaxime; from which, six isolates were ESBL-producer. Furthermore, Polymerase Chain Reaction (PCR) analysis of six ESBL- producing <em>E. coli</em> isolates showed that four isolates included <em>bla</em><sub>TEM</sub> gene, while no isolates included <em>bla</em><sub>SH</sub><sub>V</sub> gene.<br> <strong>Conclusion:</strong> In this study, multiple antibiotic resistance patterns were seen in <em>E. coli&nbsp;</em>isolates, especially ESBL patterns in <em>E. coli</em> isolated from strawberries produced in Iran.&nbsp;<br> <br> <strong>DOI:</strong> 10.18502/jfqhc.8.3.7199</span></span></div> Escherichia coli, beta-Lactamases, Drug Resistance, Microbial, Polymerase Chain Reaction, Fragaria, Iran 125 130 http://jfqhc.ssu.ac.ir/browse.php?a_code=A-10-152-13&slc_lang=en&sid=1 M.M. Soltan Dallal soltanda@sina.tums.ac.ir 0000-0002-3421-3974 Yes Division of Food Microbiology, Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran H. Abdolmaleki No Division of Food Microbiology, Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran R. Ramazanzadeh No Cellular and Molecular Research Center, Kurdistan University of Medical Sciences, Sanandaj, Iran R. Mazaheri Nezhad Fard No Food Microbiology Research Center, Tehran University of Medical Sciences, Tehran, Iran