Journal of Food Quality and Hazards Control
مجله کیفیت و کنترل مخاطرات مواد غذایی
J. Food Qual. Hazards Control
Medical Sciences
http://jfqhc.ssu.ac.ir
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admin
2345-685X
2345-6825
8
10.29252/jfqhc
14
8888
13
en
jalali
1399
12
1
gregorian
2021
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1
8
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online
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fulltext
en
Using Comet Assay for Genotoxic Evaluation of Zataria multiflora Boiss., An Iranian Endemic Plant
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<div style="text-align: justify;"><span style="font-size:14px;"><span style="font-family:Times New Roman;"><strong>Background: </strong><em>Zataria multiflora</em> Boiss. (Avishan-e Shirazi), as an Iranian endemic plant, belongs to the Lamiaceae family and may be used as a food preservative. This study aimed to detect potential genotoxic effects of <em>Z. multiflora</em> extract. <br>
<strong>Methods:</strong> Hydro-alcoholic extract of <em>Z. multiflora</em> was prepared. Human B lymphocytes were treated with 1% extract within 3 and 24 h. Sterile Phosphate Buffered Saline (PBS) and cisplatin were used as negative and positive controls, respectively. DNA damage profiles were examined using comet assay (Single Cell Gel Electrophoresis). Data were statistically analyzed by SPSS software v. 21.<br>
<strong>Results:</strong> No statistically significant (<em>p</em>=0.071) DNA damage was observed in B lymphocytes treated with either <em>Z. multiflora</em> extract or PBS after 3 and 24 h. However, there was a statistically significant difference (<em>p</em>=0.0001) between DNA damage in B lymphocytes that treated with cisplatin and <em>Z. multiflora</em> after 3 and 24 h.<br>
<strong>Conclusion:</strong> The comet assay used in the current study showed that <em>Z. multiflora </em>had no genotoxic effect.<br>
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<strong>DOI:</strong> 10.18502/jfqhc.8.1.5463</span></span></div>
Thymus Plant, Comet Assay, Mutagenicity Tests, DNA Damage, Food Safety
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http://jfqhc.ssu.ac.ir/browse.php?a_code=A-10-234-1&slc_lang=en&sid=1
Z.
Ayubi