en تخصصي Special Original article Original article <div align="justify"><font face="times new roman,times,serif" size="2"><b>Background: </b><i>Sarcocystosis </i>is one of the most widespread parasitic diseases in cattle caused by three main species of <em>Sarcocystis</em> genus. The major aim of this study was to determine the prevalence of <i>Sarcocystis </i>spp. in meat produced in Yazd, central Iran with special reference to species identification, using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP).</font><br> <font face="times new roman,times,serif" size="2">&nbsp;<b>Methods:</b> During March 2012 to May 2013, samples were randomly collected from esophagus, heart, diaphragm, intercostal muscle and tongue of 120 slaughtered cattle at Yazd, central Iran. After DNA extraction, PCR-RFLP was used for detection and identification of <i>Sarcocystis </i>spp. The statistical analysis was performed by Chi-Square test, using SPSS software (v. 16.0). </font><br> <font face="times new roman,times,serif" size="2"><b>Results:</b> The molecular analysis showed that <i>Sarcocystis </i>spp. was found in 112 of 120 (93.3%) slaughtered cattle. The prevalence of <i>S. cruzi</i>, <i>S. hirsuta</i> and <i>S. hominis</i> were 90%, 38.3% and 57.5% respectively. Among the 112 infected cattle, single and multiple (infected by more than one species) infection were seen in 45 and 67 cattle, respectively. No significant association was detected between sex, age, sample type and the prevalence of <i>Sarcocystis </i>spp. (<i>p</i>>0.05). </font><br> <font face="times new roman,times,serif" size="2"><b>Conclusion: </b>Considering its public health importance, high prevalence of <i>S. hominis</i> should be highlighted in this region. To the best of our knowledge, this study is the first of its kind to be conducted in Iran. More detailed studies are needed to describe the distribution pattern and species identification of <i>Sarcocycstis </i>spp. in other regions of Iran. </font></div> Sarcocystis, Prevalence, Molecular Diagnostic Techniques, Meat, Iran 95 101 http://jfqhc.ssu.ac.ir/browse.php?a_code=A-10-1-41&slc_lang=en&sid=1 B. Hajimohammadi No G. Eslami No A. Zohourtabar No A. Dehghani No A. Oryan No H. Pourmirzaei Tafti No F. Radouani fouzia.radouani@pasteur.ma Yes Microbiology Service, Research Department, Institut Pasteur, Casablanca, Maroc