Volume 6, Issue 4 (December 2019)                   J. Food Qual. Hazards Control 2019, 6(4): 153-161 | Back to browse issues page

XML Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Dalir Ghaffari A, Dalimi A. Molecular Identification of Toxoplasma gondii in the Native Slaughtered Cattle of Tehran Province, Iran. J. Food Qual. Hazards Control. 2019; 6 (4) :153-161
URL: http://jfqhc.ssu.ac.ir/article-1-633-en.html
Department of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran , dalimi_a@modares.ac.ir
Abstract:   (2897 Views)
Background: Toxoplasmosis, caused by Toxoplasma gondii, is a common parasitic disease, affecting almost one-third of the world’s population. It is transmitted by ingestion of food or water contaminated with oocysts excreted by cats and the consumption of raw or undercooked meat from ruminants. This study aimed at molecular characterization of T. gondii in native cattle from West of Tehran, Iran.
Methods: A total of 180 samples were collected from the cattle diaphragms (n=80) and heart muscles (n=100) from multiple slaughterhouses. The nested Polymerase Chain Reaction (PCR) assay was carried out to amplify the GRA6 gene of T. gondii. The PCR-Restriction Fragment Length Polymerase (PCR-RFLP) assay was also performed on positive samples, using Tru1I (MseI) restriction enzyme. Data were statistically analyzed using SPSS (v.15.0).
Results: T. gondii was found in 38 out of 180 (21.1%) samples. The infection rate in heart muscle samples (16.66%) was significantly (p<0.05) higher than the diaphragm samples (4.44%). The PCR-RFLP pattern by MseI enzyme showed that 13 (7.22%) samples were genotype II, while 25 (13.88%) were genotype III, having statistically meaningful difference (p<0.05). No genotype I was found in the studied isolates.
Conclusion: Based on our findings, the frequency of T. gondii was high in the study area. Therefore, educational programs need to be implemented in order to inform people about the risks of raw or undercooked meat consumption.

DOI: 10.18502/jfqhc.6.4.1993
Full-Text [PDF 714 kb]   (591 Downloads)    
Type of Study: Original article | Subject: Special
Received: 19/08/14 | Accepted: 19/10/27 | Published: 19/12/16

1. Abdoli A., Dalimi A.H., Arbabi M., Ghaffarifar F. (2014). Neuropsychiatric manifestations of latent toxoplasmosis on mothers and their offspring. The Journal of Maternal-Fetal and Neonatal Medicine. 27: 1368-1374. [DOI: 10.3109/14767058.2013.858685] [DOI:10.3109/14767058.2013.858685] [PMID]
2. Abdoli A., Dalimi A.H., Soltanghoraee H., Ghaffarifar F. (2017). Molecular detection and genotypic characterization of Toxoplasma gondii in paraffin-embedded fetoplacental tissues of women with recurrent spontaneous abortion. International Journal of Fertility and Sterility. 10: 327-336. [DOI: 10.22074/ijfs.2016.4569]
3. Ahmad N., Qayyum M. (2014). Seroprevalence and risk factors for toxoplasmosis in large ruminants in northern Punjab, Pakistan. The Journal of Infection in Developing Countries. 8: 1022-1028. [DOI: 10.3855/jidc.4405] [DOI:10.3855/jidc.4405] [PMID]
4. Ahmadpour E., Daryani A., Sharif M., Sarvi S., Aarabi M., Mizani A., Rahimi M.T., Shokri A. (2014). Toxoplasmosis in immunocompromised patients in Iran: a systematic review and meta-analysis. The Journal of Infection in Developing Countries. 8: 1503-1510. [DOI: 10.3855/jidc.4796] [DOI:10.3855/jidc.4796] [PMID]
5. Amdouni Y., Rjeibi M.R., Rouatbi M., Amairia S., Awadi S., Gharbi M. (2017). Molecular detection of Toxoplasma gondii infection in slaughtered ruminants (sheep, goats and cattle) in Northwest Tunisia. Meat Science. 133: 180-184. [DOI: 10.1016/j.meatsci.2017.07.004] [DOI:10.1016/j.meatsci.2017.07.004] [PMID]
6. Anvari D., Saadati D., Nabavi R., Eskandani M.A. (2018). Epidemiology and molecular prevalence of Toxoplasma gondii in cattle slaughtered in Zahedan and Zabol districts, South East of Iran. Iranian Journal of Parasitology. 13: 114-119.
7. Azizi H.R., Shiran B., Boroujeni A.B., Jafari M. (2014). Molecular survey of Toxoplasma gondii in sheep, cattle and meat products in Chaharmahal va Bakhtiari Province, Southwest of Iran. Iranian Journal of Parasitology. 9: 429-439.
8. Baril L., Ancelle T., Goulet V., Thulliez P., Tirard-Fleury V., Carme B. (1999). Risk factors for Toxoplasma infection in pregnancy: a case-control study in France. Scandinavian Journal of Infectious Diseases. 31: 305-309. [DOI: 10.1080/00365549950163626] [DOI:10.1080/00365549950163626] [PMID]
9. Berger-Schoch A.E., Herrmann D.C., Schares G., Muller N., Bernet D., Gottstein B., Frey C.F. (2011). Prevalence and genotypes of Toxoplasma gondii in feline faeces (oocysts) and meat from sheep, cattle and pigs in Switzerland. Veterinary Parasitology. 177: 290-297. [DOI: 10.1016/j.vetpar.2010.11.046] [DOI:10.1016/j.vetpar.2010.11.046] [PMID]
10. Chaichan P., Mercier A., Galal L., Mahittikorn A., Ariey F., Morand S., Boumédiène F., Udonsom R., Hamidovic A., Murat J.B., Sukthana Y., Dardé M.L. (2017). Geographical distribution of Toxoplasma gondii genotypes in Asia: a link with neighboring continents. Infection, Genetics and Evolution. 53:227-238. [DOI: 10.1016/j.meegid.2017.06.002] [DOI:10.1016/j.meegid.2017.06.002] [PMID]
11. Daryani A., Sarvi S., Aarabi M., Mizani A., Ahmadpour E., Shokri A., Rahimi M.T., Sharif M. (2014). Seroprevalence of Toxoplasma gondii in the Iranian general population: a systematic review and meta-analysis. Acta Tropica. 137: 185-194. [DOI: 10.1016/j.actatropica.2014.05.015] [DOI:10.1016/j.actatropica.2014.05.015] [PMID]
12. Dubey J.P. (2016). Toxoplasmosis of animals and humans. CRC press, Boca Raton. [DOI:10.1201/9781420092370]
13. Dubey J.P., Casey S.J., Zajac A.M., Wildeus S.A., Lindsay D.S., Verma S.K., Oliveira S., Kwok O.C.H., Su C. (2014). Isolation and genetic characterization of Toxoplasma gondii from alpaca (Vicugna pacos) and sheep (Ovis aries). Tropical Animal Health and Production. 46: 1503-1507. [DOI: 10.1007/s11250-014-0652-z] [DOI:10.1007/s11250-014-0652-z] [PMID]
14. Dubey J.P., Graham D.H., Dahl E., Hilali M., El-Ghaysh A., Sreekumar C., Kwok O.C.H., Shen S.K., Lehmann T. (2003). Isolation and molecular characterization of Toxoplasma gondii from chickens and ducks from Egypt. Veterinary Parasitology. 114: 89-95. [DOI: 10.1016/s0304-4017(03)00133-x] [DOI:10.1016/S0304-4017(03)00133-X]
15. Dubey J.P., Jones J.L. (2008). Toxoplasma gondii infection in humans and animals in the United States. International Journal for Parasitology. 38: 1257-1278. [DOI: 10.1016/j. ijpara.2008.03.007] [DOI:10.1016/j.ijpara.2008.03.007] [PMID]
16. Dubey J., Velmurugan G.V., Chockalingam A., Pena H.F.J., de Oliveira L.N., Leifer C.A., Gennari S.M., Bahia Oliveira L.M.G., Su C. (2008). Genetic diversity of Toxoplasma gondii isolates from chickens from Brazil. Veterinary Parasitology. 157: 299-305. [DOI: 10.1016/j.vetpar.2008.07.036] [DOI:10.1016/j.vetpar.2008.07.036] [PMID] [PMCID]
17. Foroutan-Rad M., Khademvatan S., Majidiani H.R., Aryamand S., Rahim F., Saki Malehi A. (2016a). Seroprevalence of Toxoplasma gondii in the Iranian pregnant women: a systematic review and meta-analysis. Acta Tropica. 158: 160-169. [DOI: 10.1016/j.actatropica.2016.03.003] [DOI:10.1016/j.actatropica.2016.03.003] [PMID]
18. Foroutan-Rad M., Majidiani H.R., Dalvand S., Daryani A., Kooti W., Saki J., Hedayati-Rad F., Ahmadpour E. (2016b). Toxoplasmosis in blood donors: a systematic review and meta-analysis. Transfusion Medicine Reviews. 30: 116-122. [DOI: 10.1016/j.tmrv.2016.03.002] [DOI:10.1016/j.tmrv.2016.03.002] [PMID]
19. Hashemi S. (2014). Seroprevalence of toxoplasmosis in cattle, sheep and goat, using ELISA and Indirect Immunoflorescent Antibody (IFA) methods in Lorestan province. Journal of Large Animal Clinical Science Research. 7 : 49-55. [Persian with English abstract]
20. Howe D.K., Sibley L.D. (1995). Toxoplasma gondii comprises three clonal lineages: correlation of parasite genotype with human disease. Journal of Infectious Diseases. 172: 1561-1566. [DOI: 10.1093/infdis/172.6.1561] [DOI:10.1093/infdis/172.6.1561] [PMID]
21. Khan A., Su C., German M., Storch G.A., Clifford D.B., Sibley L. D. (2005). Genotyping of Toxoplasma gondii strains from immunocompromised patients reveals high prevalence of type I strains. Journal of Clinical Microbiology. 43: 5881-5887. [DOI: 10.1128/JCM.43.12.5881-5887.2005] [DOI:10.1128/JCM.43.12.5881-5887.2005] [PMID] [PMCID]
22. Lahmar I., Lachkhem A., Slama D., Sakly W., Haouas N., Gorcii M., Pfaff A.W., Candolfi E., Babba H. (2015). Prevalence of toxoplasmosis in sheep, goats and cattle in Southern Tunisia. Journal of Bacteriology and Parasitology. 6: 1000245. [DOI: 10.4172/2155-9597.1000245] [DOI:10.4172/2155-9597.1000245]
23. Lopes A.P., Dubey J.P., Neto F., Rodrigues A., Martins T., Rodrigues M., Cardoso L. (2013). Seroprevalence of Toxoplasma gondii infection in cattle, sheep, goats and pigs from the North of Portugal for human consumption. Veterinary Parasitology. 193: 266-269. [DOI: 10.1016/j. vetpar.2012.12.001] [DOI:10.1016/j.vetpar.2012.12.001] [PMID]
24. Lopes A.P., Vilares A., Neto F., Rodrigues A., Martins T., Ferreira I., Gargate M.J., Rodrigues M., Cardoso L. (2015). Genotyping characterization of Toxoplasma gondii in cattle, sheep, goats and swine from the North of Portugal. Iranian Journal of Parasitology. 10: 465-472.
25. MacPherson J.M., Gajadhar A.A. (1993). Sensitive and specific polymerase chain reaction detection of Toxoplasma gondii for veterinary and medical diagnosis. Canadian Journal of Veterinary Research. 57: 45-48.
26. Mahami-Oskouei M., Moradi M., Fallah E., Hamidi F., Akbari N.A.R. (2017). Molecular detection and genotyping of Toxoplasma gondii in chicken, beef, and lamb meat consumed in Northwestern Iran. Iranian Journal of Parasitology. 12: 38-45.
27. Montoya J.G., Liesenfeld O. (2004). Toxoplasmosis. The Lancet. 363: 1965-1976. [DOI: 10.1016/S0140-6736(04)16412-X] [DOI:10.1016/S0140-6736(04)16412-X]
28. Payne S., Ellis J. (1996). Detection of Neospora caninum DNA by the polymerase chain reaction. International Journal for Parasitology. 26: 347-351. [DOI: 10.1016/0020-7519(96)00030-6] [DOI:10.1016/0020-7519(96)00030-6]
29. Rahdar M., Samarbaf-Zadeh A., Arab L. (2012). Evaluating the prevalence of Toxoplasma gondii in meat and meat products in Ahvaz by PCR method. Jundishapur Journal of Microbiology. 5: 570-573. [DOI:10.5812/jjm.4280] [DOI:10.5812/jjm.4280]
30. Rahimi M.T., Daryani A., Sarvi S., Shokri A., Ahmadpour E., Teshnizi S.H., Mizani A., Sharif M. (2015). Cats and Toxoplasma gondii: a systematic review and meta-analysis in Iran. Onderstepoort Journal of Veterinary Research. 82: 01-10. [DOI: 10.4102/OJVR.V82I1.823] [DOI:10.4102/ojvr.v82i1.823] [PMID] [PMCID]
31. Robert-Gangneux F., Dardé M.L. (2012). Epidemiology of and diagnostic strategies for toxoplasmosis. Clinical Microbiology Reviews. 25: 264-296. [DOI: 10.1128/CMR.05013-11] [DOI:10.1128/CMR.05013-11] [PMID] [PMCID]
32. Saadatnia G., Golkar M. (2012). A review on human toxoplasmosis. Scandinavian Journal of Infectious Diseases. 44: 805-814. [DOI: 10.3109/00365548.2012.693197] [DOI:10.3109/00365548.2012.693197] [PMID]
33. Sarvi S., Daryani A., Rahimi M.T., Aarabi M., Shokri A., Ahmadpour E., Mizani A., Sharif M. (2015). Cattle toxoplasmosis in Iran: a systematic review and meta-analysis. Asian Pacific Journal of Tropical Medicine. 8: 120-126. [DOI: 10.1016/S1995-7645(14)60301-1] [DOI:10.1016/S1995-7645(14)60301-1]
34. Shariat Bahadori E., Dalir Ghaffari A., Namroodi S., Mosavipour S.S., Sadraei J. (2019). Electrochemiluminesence epidemiol-ogic detection of Toxoplasma gondii infection in pregnant women with direct and indirect diagnostic techniques (ELISA avidity plus biochemical assay), Tehran, Iran. International Journal of Epidemiologic Research. 6: 49-54. [DOI: 10.15171/ ijer.2019.09] [DOI:10.15171/ijer.2019.09]
35. Sharif M., Amouei A., Sarvi S., Mizani A., Aarabi M., Hosseini S.A., Daryani A. (2017). Genetic diversity of Toxoplasma gondii isolates from ruminants: a systematic review. International Journal of Food Microbiology. 258: 38-49. [DOI: 10.1016/j.ijfoodmicro.2017.07.007] [DOI:10.1016/j.ijfoodmicro.2017.07.007] [PMID]
36. Sharif M., Sarvi S., Shokri A., Hosseini Teshnizi S., Rahimi M.T., Mizani A., Ahmadpour E., Daryani A. (2015). Toxoplasma gondii infection among sheep and goats in Iran: a systematic review and meta-analysis. Parasitology Research. 114: 1-16. [DOI: 10.1007/s00436-014-4176-2] [DOI:10.1007/s00436-014-4176-2] [PMID]
37. Sibley L.D., Khan A., Ajioka J.W., Rosenthal B.M. (2009). Geneticdiversity of Toxoplasma gondii in animals and humans. Philosophical Transactions of the Royal Society of London Series B, Biological Sciences. 364: 2749-2761. [DOI: 10.1098/rstb.2009.0087] [DOI:10.1098/rstb.2009.0087] [PMID] [PMCID]
38. Weiss L.M., Kim K. (2000). The development and biology of bradyzoites of Toxoplasma gondii. Frontiers in Bioscience: a Journal and Virtual Library. 5: D391-D405. [DOI:10.2741/A521]

Add your comments about this article : Your username or Email:

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2022 CC BY-NC 4.0 | Journal of food quality and hazards control

Designed & Developed by : Yektaweb