Volume 9, Issue 3 (September 2022)
J. Food Qual. Hazards Control 2022, 9(3): 160-168 |
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Das S, Uddin M, Haque M, Chakraborty D, Mostafa M, Hasnaine A, et al . Hydroxymethylfurfural Content and Sugar Profile of Honey Available in Bangladesh Using Validated HPLC-PDA and HPLC-RID. J. Food Qual. Hazards Control 2022; 9 (3) :160-168
URL: http://jfqhc.ssu.ac.ir/article-1-1024-en.html
URL: http://jfqhc.ssu.ac.ir/article-1-1024-en.html
S. Das * 
, M.N. Uddin , M.S. Haque , D. Chakraborty , M. Mostafa , A. Hasnaine , S.K. Das , M. Uddin
, M.N. Uddin , M.S. Haque , D. Chakraborty , M. Mostafa , A. Hasnaine , S.K. Das , M. Uddin
Bangladesh Council of Scientific and Industrial Research (BCSIR) Chattogram Laboratories, Chattogram-4,220, Bangladesh , sumanbcsir@gmail.com
Abstract: (891 Views)
Background: Honey has a lot of reputation because of its supposed medicinal properties. In this study, Hydroxymethylfurfural (HMF), sugars, and Fructose/Glucose ratio of honey in Bangladesh were assessed for adulteration and authenticity evaluation.
Methods: Seventy honey samples collected from different districts of Bangladesh were analyzed by High Performance Liquid Chromatography (HPLC) for HMF content and sugar profile. The samples were prepared by using Carrez I and Carrez II prior to injecting into HPLC. The samples were then filtered through syringe filter and taken in 1.5 ml vial for injecting into the HPLC system.
Results: HMF values were ranging from 1.41 mg/kg to 2,063.90 mg/kg. The Limit of Detection (LOD) and Limit of Quantification (LOQ) was found 0.10 mg/kg and 0.33 mg/kg with R2=0.9994. The average values of fructose, glucose, and sucrose were in the range of 14.75-52.44%, 8.19-42.63%, and 0.10-21.12%, respectively. From validation parameters, LOD values for fructose, glucose, and sucrose were 0.003, 0.008, and 0.004%, respectively; and LOQ values were 0.01, 0.028, and 0.015%, respectively with an excellent linearity with R2 for fructose=1.0, glucose=0.9999, and sucrose=1.0.
Conclusion: Some samples had higher HMF content which may be due to the storage time was increased and improper processing with high temperature or adulteration by High Fructose Corn Syrup (HFCS), sugar cane syrup, rice syrups or rice molasses. The sugar profiles showed that the most of honey samples were nectar honeys.
DOI: 10.18502/jfqhc.9.3.11154
Methods: Seventy honey samples collected from different districts of Bangladesh were analyzed by High Performance Liquid Chromatography (HPLC) for HMF content and sugar profile. The samples were prepared by using Carrez I and Carrez II prior to injecting into HPLC. The samples were then filtered through syringe filter and taken in 1.5 ml vial for injecting into the HPLC system.
Results: HMF values were ranging from 1.41 mg/kg to 2,063.90 mg/kg. The Limit of Detection (LOD) and Limit of Quantification (LOQ) was found 0.10 mg/kg and 0.33 mg/kg with R2=0.9994. The average values of fructose, glucose, and sucrose were in the range of 14.75-52.44%, 8.19-42.63%, and 0.10-21.12%, respectively. From validation parameters, LOD values for fructose, glucose, and sucrose were 0.003, 0.008, and 0.004%, respectively; and LOQ values were 0.01, 0.028, and 0.015%, respectively with an excellent linearity with R2 for fructose=1.0, glucose=0.9999, and sucrose=1.0.
Conclusion: Some samples had higher HMF content which may be due to the storage time was increased and improper processing with high temperature or adulteration by High Fructose Corn Syrup (HFCS), sugar cane syrup, rice syrups or rice molasses. The sugar profiles showed that the most of honey samples were nectar honeys.
DOI: 10.18502/jfqhc.9.3.11154
Keywords: Honey, 5-Hydroxymethylfurfural, Chromatography, High Pressure Liquid, Fraud, Sucrose, Bangladesh
Type of Study: Original article |
Subject:
Special
Received: 21/11/20 | Accepted: 22/04/05 | Published: 22/09/24
Received: 21/11/20 | Accepted: 22/04/05 | Published: 22/09/24
References
1. Aazza S., Lyoussi B., Antunes D., Miguel M.G. (2014). Physicochemical characterization and antioxidant activity of 17 commercial Moroccan honeys. International Journal of Food Science and Nutrition. 65: 449-457. [DOI: 10.3109/ 09637486.2013.873888] [DOI:10.3109/09637486.2013.873888] [PMID]
2. Abdallah E.M., Hamed A.I. (2019). Screening for antibacterial activity of two jujube honey samples collected from Saudi Arabia. Journal of Apitherapy. 5: 6-9. [DOI: 10.5455/ja. 20190120035814] [DOI:10.5455/ja.20190120035814]
3. Alghamdi B.A., Alshumrani E.S., Saeed M.S.B., Rawas G.M., Alharthi N.T., Baeshen M.N., Helmi N.M., Alam M.Z., Suhail M. (2020). Analysis of sugar composition and pesticides using HPLC and GC-MS techniques in honey samples collected from Saudi Arabian markets. Saudi Journal of Biological Sciences. 27: 3720-3726. [DOI: 10.1016/j.sjbs.2020.08.018] [DOI:10.1016/j.sjbs.2020.08.018] [PMID] [PMCID]
4. Ali M.S., Fahad-Bin-Quader., Islam A., Ahmed S., Siddiqua A., Akter T. (2018). Qualitative evaluation of honey available in Bangladeshi markets. International Journal of Natural and Social Sciences. 5: 20-27.
5. Aljohar H.I., Maher H.M., Albaqami J., Al-Mehaizie M., Orfali R., Orfali R., Alrubia S. (2018). Physical and chemical screening of honey samples available in the Saudi market: an important aspect in the authentication process and quality assessment. Saudi Pharmaceutical Journal. 26: 932-942. [DOI: 10.1016/ j.jsps.2018.04.013] [DOI:10.1016/j.jsps.2018.04.013] [PMID] [PMCID]
6. Amiry S., Esmaiili M., Alizadeh M. (2017). Classification of adulterated honeys by multivariate analysis. Food Chemistry. 224: 390-397. [DOI: 10.1016/j.foodchem.2016.12.025] [DOI:10.1016/j.foodchem.2016.12.025] [PMID]
7. Bastos D.M., Monaro É., Siguemoto É., Séfora M. (2012). Maillard reaction products in processed food: pros and cons. In: Valdez B. (Editors). Food industrial processes - methods and equipment. InTech, Rijeka, Croatia. pp: 281-300. [DOI: 10.5772/ 31925]
8. Cengiz M.F., Durak M.Z., Ozturk M. (2014). In-house validation for the determination of honey adulteration with plant sugars (C4) by isotope ratio mass spectrometry (IR-MS). LWT - Food Science and Technology. 57: 9-15. [DOI: 10.1016/j.lwt.2013. 12.032] [DOI:10.1016/j.lwt.2013.12.032]
9. Codex Alimentarius Commission. (2001). Revised codex standard for Honey. Codex standard 12-1981. 11: 1-8.
10. Cozmuta A.M., Cozmuta L.M., Varga C., Marian M., Peter A. (2011). Effect of thermal processing on quality of polyfloral honey. Romanian Journal of Food Science. 1: 45-52.
11. De Almeida A.M.M., Oliveira M.B.S., Da Costa J.G., Valentim I.B., Goulart M.O.F. (2016). Antioxidant capacity, physicochemical and floral characterization of honeys from the northeast of Brazil. Revista Virtual de Quimica. 8: 57-77. [DOI: 10.5935/1984-6835.20160005] [DOI:10.5935/1984-6835.20160005]
12. De Almeida-Muradian L.B., Stramm K.M., Horita A., Barth O.M., De Freitas A.D.S., Estevinho L.M. (2013). Comparative study of the physicochemical and palynological characteristics of honey from Melipona subnitida and Apis mellifera. International Journal of Food Science and Technology. 48: 1698-1706. [DOI: 10.1111/ijfs.12140] [DOI:10.1111/ijfs.12140]
13. Dobre I., Georgescu L.A., Alexe P., Escuredo O., Seijo M.C. (2012). Rheological behavior of different honey types from Romania. Food Research International. 49: 126-132. [DOI: 10.1016/j.foodres.2012.08.009] [DOI:10.1016/j.foodres.2012.08.009]
14. Elhamdaoui O., El Orche A., Cheikh A., Mojemmi B., Nejjari R., Bouatia M. (2020). Development of fast analytical method for the detection and quantification of honey adulteration using vibrational spectroscopy and chemometrics tools. Journal of Analytical Methods in Chemistry. 2020. [DOI: 10.1155/ 2020/8816249] [DOI:10.1155/2020/8816249] [PMID] [PMCID]
15. El Sohaimy S.A., Masry S.H.D., Shehata M.G. (2015). Physicochemical characteristics of honey from different origins. Annals of Agricultural Science. 60: 279-287. [DOI: 10.1016/j. aoas.2015.10.015] [DOI:10.1016/j.aoas.2015.10.015]
16. Eslamizad S., Kobarfard F., Tabib K., Yazdanpanah H., Salamzadeh J. (2020). Development of a sensitive and rapid method for determination of acrylamide in bread by LC-MS/MS and analysis of real samples in Iran IR. Iranian Journal of Pharmaceutical Research. 19: 413-423. [DOI: 10.22037/ijpr. 2019.111994.13474]
17. European :union:. (2014). Directive 2014/63/EU of the European parliament and of the council of 15 May 2014 amending council directive 2001/110/EC relating to honey. Official Journal of the European :union:. L 164: 1-5. URL: https://eur-lex.europa.eu/legal-content/EN/TXT/PDF/?uri=CELEX:32014L0063&from=EN.
18. Ghramh H.A., Khan K.A., Zubair A., Ansari M.J. (2020). Quality evaluation of Saudi honey harvested from the Asir province by using high-performance liquid chromatography (HPLC). Saudi Journal of Biological Sciences. 27: 2097-2105. [DOI: 10.1016/j.sjbs.2020.04.009] [DOI:10.1016/j.sjbs.2020.04.009] [PMID] [PMCID]
19. Habib H.M., Al Meqbali F.T., Kamal H., Souka U.D., Ibrahim W.H. (2014). Physicochemical and biochemical properties of honeys from arid regions. Food Chemistry. 153: 35-43. [DOI: 10.1016/j.foodchem.2013.12.048] [DOI:10.1016/j.foodchem.2013.12.048] [PMID]
20. Islam A., Khalil I., Islam N., Moniruzzaman M., Mottalib A., Sulaiman S.A., Gan S.H. (2012). Physicochemical and antioxidant properties of Bangladeshi honeys stored for more than one year. BMC Complementary and Alternative Medicine. 12: 177. [DOI: 10.1186/1472-6882-12-177] [DOI:10.1186/1472-6882-12-177] [PMID] [PMCID]
21. Islam S., Jothi J.S., Islam M., Zubair A. (2014). Antioxidant and physico-chemical properties of litchi honey procured from Gazipur and Tangail district, bangladesh. Journal of Entomology and Zoology Studies. 2: 207-211.
22. Jandrić Z., Frew R.D., Fernandez-Cedi L.N., Cannavan A. (2017). An investigative study on discrimination of honey of various floral and geographical origins using UPLC QToF MS and multivariate data analysis. Food Control. 72: 189-197. [DOI: 10.1016/j.foodcont.2015.10.010] [DOI:10.1016/j.foodcont.2015.10.010]
23. Karabagias I.K., Louppis A.P., Kontakos S., Drouza C., Papastephanou C. (2018). Characterization and botanical differentiation of monofloral and multifloral honeys produced in Cyprus, Greece, and Egypt using physicochemical parameter analysis and mineral content in conjunction with supervised statistical techniques. Journal of Analytical Methods in Chemistry. 2018. [DOI: 10.1155/2018/7698251] [DOI:10.1155/2018/7698251] [PMID] [PMCID]
24. Khan K.A., Al-Ghamdi A.A., Ansari M.J. (2016). The characterization of blossom honeys from two provinces of Pakistan. Italian Journal of Food Science. 28: 625-638. [DOI 10.14674/1120-1770/ijfs.v268]
25. Kirs E., Pall R., Martverk K., Laos K. (2011). Physicochemical and melissopalynological characterization of Estonian summer honeys. Procedia Food Science. 1: 616-624. [DOI: 10.1016/j.profoo.2011.09.093] [DOI:10.1016/j.profoo.2011.09.093]
26. Linkon M.R., Prodhan U.K., Elahi T., Talukdar J., Alim A. (2015). Comparative analysis of the physico-chemical and antioxidant properties of honey available in Tangail, Bangladesh. Universal Journal of Food and Nutrition Science. 3: 19-22. [DOI: 10.13189/ujfns.2015.030103] [DOI:10.13189/ujfns.2015.030103]
27. Ma Y., Zhang B., Li H., Li Y., Hu J., Li J., Wang H., Deng Z. (2017). Chemical and molecular dynamics analysis of crystallization properties of honey. International Journal of Food Properties. 20: 725-733. [DOI: 10.1080/10942912.2016. 1178282] [DOI:10.1080/10942912.2016.1178282]
28. Mehryar L., Esmaiili M., Hassanzadeh A. (2013). Evaluation of some physicochemical and rheological properties of Iranian honeys and the effect of temperature on its viscosity. American-Eurasian Journal of Agricultural and Environmental Sciences. 13: 807-819. [DOI: 10.5829/idosi.aejaes.2013.13.06. 1971]
29. Meo S.A., Al-Asiri S.A., Mahesar A.L., Ansari M.J. (2017). Role of honey in modern medicine. Saudi Journal of Biological Sciences. 24: 975-978. [DOI: 10.1016/j.sjbs.2016.12.010] [DOI:10.1016/j.sjbs.2016.12.010] [PMID] [PMCID]
30. Mohammed M.E.A., Alfifi A., Aalmudaw A., Alfaifi M.Y., Elbehairi S.E.I., Al-bushnaq H.A. (2017). Some physiochemical properties of Acacia honey from different altitudes of the Asir region in southern Saudi Arabia. Czech Journal of Food Science. 35: 321-327. [DOI: 10.17221/428/2016-CJFS] [DOI:10.17221/428/2016-CJFS]
31. Rodríguez Flores M.S., Escuredo Pérez O., Seijo Coello M.C. (2014). Characterization of Eucalyptus globulus honeys produced in the Eurosiberian area of the Iberian Peninsula. International Journal of Food Properties. 17: 2177-2191. [DOI: 10.1080/10942912.2013.790050] [DOI:10.1080/10942912.2013.790050]
32. Samarghandian S., Farkhondeh T., Samini F. (2017). Honey and health: a review of recent clinical research. Pharmacognosy Research. 9: 121-127. [DOI: 10.4103/0974-8490.204647]
33. Shapla U.M., Solayman., Alam N., Khalil I., Gan S.H. (2018). 5-Hydroxymethylfurfural (HMF) levels in honey and other food products: effects on bees and human health. Chemistry Central Journal. 12: 35. [DOI: 10.1186/s13065-018-0408-3] [DOI:10.1186/s13065-018-0408-3] [PMID] [PMCID]
34. Sobrino-Gregorio L., Vargas M., Chiralt A., Escriche I. (2017). Thermal properties of honey as affected by the addition of sugar syrup. Journal of Food Engineering. 213: 69-75. [DOI: 10.1016/j.jfoodeng.2017.02.014] [DOI:10.1016/j.jfoodeng.2017.02.014]
35. Uran H., Aksu F., Altiner D.D. (2017). A research on the chemical and microbiological qualities of honeys sold in Istanbul. Food Science and Technology. 37: 30-33. [10.1590/1678-457x.32016] [DOI:10.1590/1678-457x.32016]
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