Volume 10, Issue 2 (June 2023)
J. Food Qual. Hazards Control 2023, 10(2): 76-83 |
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Okike O, Daramola S, Nwachukwu F, Inana M, Nwebor E, Aneke C. Microbial and Fungal Contamination of Different Dried Cocoyam Flakes during Storage. J. Food Qual. Hazards Control 2023; 10 (2) :76-83
URL: http://jfqhc.ssu.ac.ir/article-1-1074-en.html
URL: http://jfqhc.ssu.ac.ir/article-1-1074-en.html
Nigerian Stored Products Research Institute, Mile 4, Ikwere Road, Rumueme, P.M.B. 5,063, Port Harcourt, Nigeria , Okike.obinnaodii108@gmail.com
Abstract: (483 Views)
Background: Contamination of food by microorganisms from the processing to the consumption stage has become a major health concern in this era, where a better approach for the elongation of food shelf life is explored. This study aims to evaluate the microbial and fungal contamination of different dried cocoyam flakes during storage.
Methods: The cocoyam (Colocasia esculenta) samples were properly cooked, peeled, sliced into thin shapes, and dried to constant weights. The drying methods used were sun, hot-air oven, and Nigerian Stored Products Research Institute (NSPRI) multi-crop dryer; samples were divided into three groups A, B, and C, respectively, and the dried samples were stored for three months. Moisture Content (MC) was conducted using the standard method. Colonial morphology, Gram staining, and biochemical test were used to identify and characterize microorganisms. Statistical analyses were performed using SPSS 20.0.
Results: There was a significant increase in the MC of all the samples after the storage duration. The sun-dried sample had the highest MC (13.60%) while the oven-dried sample had the lowest MC (10.82%). The sun-dried samples had the highest viable bacteria count (7.2×105 Colony Forming Unit (CFU)/g) and the oven-dried sample had the lowest count (5.1×105 CFU/g). The four bacterial isolates identified were Bacillus subtilis, Pseudomonas aeruginosa, Micrococcus luteus, and Staphylococcus aureus. The heterotrophic fungal number was the highest (0.5×103 CFU/g) regarding sun-dried samples, whereas the samples dried with NSPRI multi-crop dryer and oven-dried samples had the same value (0.3×103 CFU/g). Aspergillus flavus, A. niger, Rhizopus, Penicillium, and Mucor were isolated from the samples. Rhyzopertha dominica was identified in sun-dried samples.
Conclusion: The increase of MC and the nature of the storage material may have contributed to high bacterial and fungal counts of stored dried cocoyam flakes, especially sun-dried ones, thereby exposing consumers to potential health risks.
DOI: 10.18502/jfqhc.10.2.12670
Methods: The cocoyam (Colocasia esculenta) samples were properly cooked, peeled, sliced into thin shapes, and dried to constant weights. The drying methods used were sun, hot-air oven, and Nigerian Stored Products Research Institute (NSPRI) multi-crop dryer; samples were divided into three groups A, B, and C, respectively, and the dried samples were stored for three months. Moisture Content (MC) was conducted using the standard method. Colonial morphology, Gram staining, and biochemical test were used to identify and characterize microorganisms. Statistical analyses were performed using SPSS 20.0.
Results: There was a significant increase in the MC of all the samples after the storage duration. The sun-dried sample had the highest MC (13.60%) while the oven-dried sample had the lowest MC (10.82%). The sun-dried samples had the highest viable bacteria count (7.2×105 Colony Forming Unit (CFU)/g) and the oven-dried sample had the lowest count (5.1×105 CFU/g). The four bacterial isolates identified were Bacillus subtilis, Pseudomonas aeruginosa, Micrococcus luteus, and Staphylococcus aureus. The heterotrophic fungal number was the highest (0.5×103 CFU/g) regarding sun-dried samples, whereas the samples dried with NSPRI multi-crop dryer and oven-dried samples had the same value (0.3×103 CFU/g). Aspergillus flavus, A. niger, Rhizopus, Penicillium, and Mucor were isolated from the samples. Rhyzopertha dominica was identified in sun-dried samples.
Conclusion: The increase of MC and the nature of the storage material may have contributed to high bacterial and fungal counts of stored dried cocoyam flakes, especially sun-dried ones, thereby exposing consumers to potential health risks.
DOI: 10.18502/jfqhc.10.2.12670
Type of Study: Original article |
Subject:
Special
Received: 22/09/10 | Accepted: 23/02/18 | Published: 23/06/28
Received: 22/09/10 | Accepted: 23/02/18 | Published: 23/06/28
References
1. Abrego N., Crosier B., Somervuo P., Ivanova N., Abrahamyan A., Abdi A., Hämäläinen K., Junninen K., Maunula M., Purhonen J., Ovaskainen O. (2020). Fungal communities decline with urbanization-more in air than in soil. The International Society for Microbial Ecology Journal. 14: 2806-2815. [DOI: 10.1038/s41396-020-0732-1] [DOI:10.1038/s41396-020-0732-1] [PMID] [PMCID]
2. Afolabi I.S. (2014). Moisture migration and bulk nutrients interaction in a drying food system: a review. Food and Nutrition Sciences. 5: 692-714. [DOI: 10.4236/fns.2014.58080] [DOI:10.4236/fns.2014.58080]
3. Ali Z., Sana S., Sheikh A.A., Maheen Z. (2022). Molecular characterization of toxigenic Aspergillus flavus isolated from sick broiler lungs and risk factors analysis. Pakistan Veterinary Journal. 42: 194-200. [DOI: 10.29261/pakvetj/2022.037]
4. Alp D., Bulantekin Ö. (2021). The microbiological quality of various foods dried by applying different drying methods: a review. European Food Research and Technology. 247: 1333-1343. [DOI: 10.1007/s00217-021-03731-z] [DOI:10.1007/s00217-021-03731-z] [PMID] [PMCID]
5. Anyanwu A.G., Orji N.O., Agwu C.O., Nwaobia H.O., Isima P.C., Obasi T.O., Anucha C., Ikpa S.C., Chidi P.C. (2023). Bacteria and fungi associated with the deterioration of cocoyam Colocasia esculenta (Taro). World Journal of Advanced Research and Reviews. 18: 841-847. [DOI: 10.30574/wjarr.2023.18.1.0600] [DOI:10.30574/wjarr.2023.18.1.0600]
6. AOAC. (2019). Offcial methods of analysis of AOAC international. 21st edition. AOAC International, Washington D.C, USA.
7. Beuchat L.R., Komitopoulou E., Beckers H., Betts R.P., Bourdichon F., Fanning S., Joosten N.M., Kuile B.H.T. (2013). Low--water activity foods: increased concern as vehicles of foodborne pathogens. Journal of Food Protection. 76: 150-172. [DOI: 10.4315/0362-028X.JFP-12-211] [DOI:10.4315/0362-028X.JFP-12-211] [PMID]
8. Cechin C.D.F., Carvalho G.G., Bastos C.P., Kabuki D.Y. (2022). Cronobacter spp. in foods of plant origin: occurrence, contamination routes, and pathogenic potential. Critical Reviews in Food Science and Nutrition. 1-15. [DOI: 10.1080/10408398.2022.2101426] [DOI:10.1080/10408398.2022.2101426] [PMID]
9. Chitravathi K., Chauhan O.P., Raju P.S. (2015). Influence of modified atmosphere packaging on shelf-life of green chillies (Capsicum annuum L.). Food Packaging and Shelf Life. 4: 1-9. [DOI: 10.1016/j.fpsl.2015.02.001] [DOI:10.1016/j.fpsl.2015.02.001]
10. Deng L.-Z., Mujumdar A.S., Zhang Q., Yang X.-H., Wang J., Zheng Z.-A., Gao Z.-J., Xiao H.-W. (2019). Chemical and physical pretreatments of fruits and vegetables: effects on drying characteristics and quality attributes - a comprehensive review. Critical Reviews in Food Science and Nutrition. 59: 1408-1432. [DOI: 10.1080/10408398.2017.1409192] [DOI:10.1080/10408398.2017.1409192] [PMID]
11. Douglas S.I., Robinson V.K. (2019). Indoor microbiological air quality in some wards of a tertiary health institution in Port Harcourt, Nigeria. IOSR Journal of Pharmacy and Biological Sciences. 14: 44-50. [DOI: 10.9790/3008-1401034450]
12. Elik A., Yanik D.K., Istanbullu Y., Guzelsoy N.A., Yavuz A., Gogus F. (2019). Strategies to reduce post-harvest losses for fruits and vegetables. International Journal of Scientific and Technological Research. 5: 29-39. [DOI: 10.7176/JSTR/5-3-04] [DOI:10.7176/JSTR/5-3-04]
13. El-Prince E., Sayed A.M., Ewida R.M. (2023). Coliform contamination of marketable milk sold in new valley governorate, Egypt. Journal of Advanced Veterinary Research. 13: 241-245.
14. Estelle L.Y.L., Joelle T.D.M., Azize O. (2019). Integrated pest management of the yam chip beetle Dinoderus porcellus lesne (Coleoptera: Bostrichidae): current status and future prospects. Trends in Integrated Insect Pest Management. [DOI: 10.5772/intechopen.87926] [DOI:10.5772/intechopen.87926]
15. Garcia S.L. (2016). Mixed cultures as model communities: hunting for ubiquitous microorganisms, their partners, and interactions. Aquatic Microbial Ecology. 77: 79-85. [DOI: 10.3354/ame01789] [DOI:10.3354/ame01789]
16. Gavahian M., Chu Y.-H., Mousavi Khaneghah A., Barba F.J., Misra N.N. (2018). A critical analysis of the cold plasma induced lipid oxidation in foods. Trends in Food Science and Technology. 77: 32-41. [DOI: 10.1016/j.tifs.2018.04.009] [DOI:10.1016/j.tifs.2018.04.009]
17. Guo W., Tang X., Cui S., Zhang Q., Zhao J., Mao B., Zhang H. (2023). Recent advance in quality preservation of non-thermal preservation technology of fresh mushroom: a review. Critical Reviews in Food Science and Nutrition. 27: 1-17. [DOI: 10.1080/10408398.2023.2193636] [DOI:10.1080/10408398.2023.2193636]
18. Hammond S.T., Brown J.H., Burger J.R., Flanagan T.P., Fristoe T.S., Mercado-Silva N., Nekola J.C., Okie J.G. (2015). Food spoilage, storage, and transport: implications for a sustainable future. BioScience. 65: 758-768. [DOI: 10.1093/biosci/biv081] [DOI:10.1093/biosci/biv081]
19. Ilondu E.M. (2017). Effects of different drying methods on the mycoflora associated with cocoyam (Colocasia esculenta (L) Schott) chips (Achicha) in storage. Journal of Advances in Biology and Biotechnology. 11: 1-7. [DOI: 10.9734/JABB/2017/31035] [DOI:10.9734/JABB/2017/31035]
20. Isah M.D., Ayertey J.N., Ukeh D.A., Umoetok S.B.A. (2012). Damage and weight loss to dried chips of cassava, cocoyam, yam and plantain exposed to Prostephanus truncatus (Horn) (Coleoptera: Bostrichidae) over three different time durations. Journal of Entomology. 9: 137-145. [DOI: 10.3923/je. 2012.137.145] [DOI:10.3923/je.2012.137.145]
21. James M., Owino W., Imathiu S. (2022). Microbial contamination and occurrence of aflatoxins in processed baobab products in Kenya. International Journal of Food Science. 2022. [DOI: 10.1155/2022/2577222] [DOI:10.1155/2022/2577222] [PMID] [PMCID]
22. Jovanovic J., Ornelis V.F.M., Madder A., Rajkovic A. (2021). Bacillus cereus food intoxication and toxicoinfection. Comprehensive Reviews in Food Science and Food Safety. 20: 3719-3761. [DOI: 10.1111/1541-4337.12785] [DOI:10.1111/1541-4337.12785] [PMID]
23. Kharel N., Palni U., Tamang J.P. (2016). Microbiological assessment of ethnic street foods of the Himalayas. Journal of Ethnic Foods. 3: 235-241. [DOI: 10.1016/j.jef.2016.01.001] [DOI:10.1016/j.jef.2016.01.001]
24. Kidd S., Halliday C., Alexiou H., Ellis D. (2016). Descriptions of medical fungi. 3rd edition. CutCut Digital, Paris.
25. Kpormon L., Douglas S. (2018). Comparative ecotoxicological assay of e-waste (phone batteries) on some aquatic micro flora. Journal of Advances in Biology and Biotechnology. 17: 1-10. [DOI: 10.9734/JABB/2018/40421] [DOI:10.9734/JABB/2018/40421]
26. Lebot V., Malapa R., Jung M. (2013). Use of NIRS for rapid prediction of total N, minerals, sugars and starch in tropical root and tuber crops. New Zealand Journal of Crop and Horticultural Science. 41: 144-153. [DOI: 10.1080/01140671.2013.798335] [DOI:10.1080/01140671.2013.798335]
27. Liu S., Roopesh M.S., Tang J., Wu Q., Qin W. (2022). Recent development in low-moisture foods: microbial safety and thermal process. Food Research International. 155: 111072. [DOI: 10.1016/j.foodres.2022.111072] [DOI:10.1016/j.foodres.2022.111072] [PMID]
28. Makinde O.M., Ayeni K.I., Sulyok M., Krska R., Adeleke R.A., Ezekiel C.N. (2020). Microbiological safety of ready-to-eat foods in low- and middle-income countries: a comprehensive 10-year (2009 to 2018) review. Comprehensive Reviews in Food Science and Food Safety. 19: 703-732. [DOI: 10.1111/1541-4337.12533] [DOI:10.1111/1541-4337.12533] [PMID]
29. Møretrø T., Langsrud S. (2017). Residential bacteria on surfaces in the food industry and their implications for food safety and quality. Comprehensive Reviews in Food Science and Food Safety. 16: 1022-1041. [DOI: 10.1111/1541-4337.12283] [DOI:10.1111/1541-4337.12283] [PMID]
30. Ojewumi M.E., Omoleye A.J., Ajayi A.A. (2016). The study of the effect of moisture content on the biochemical deterioration of stored fermented Parkia biglobosa seeds. Open Journal of Engineering Research and Technology. 1: 14-22.
31. Okwu G.I., Akpe A.R., Osawaru A.A. (2021) Shelf stability of processed cocoyam flour during storage at room temperature (28.0 ± 2°C) for a period of four months. African Journal of Microbiology Research. 15: 272-277. [DOI: 10.5897/AJMR2020.9404] [DOI:10.5897/AJMR2020.9404]
32. Omojasola P.F., Sanu F.T. (2013). Microbiological quality assessment of dried yam chips (Dioscorea rotundata) during Storage. Journal of Microbiology, Biotechnology and Food Sciences. 3: 208-213.
33. Plotnikova I.V., Magomedov G.O., Zharkova I.M., Miroshnichenko E.N., Plotnikov V.E. (2022). Jelly formulated with different carbohydrate profiles: quality evaluation. Foods and Raw Materials. 10: 262-273. [DOI: 10.21603/2308-4057-2022-2-535] [DOI:10.21603/2308-4057-2022-2-535]
34. Prescott L.M., Harley J.P., Klein D.A. (2011). Microbiology. 8th edition. WMC Brown, London.
35. Ramawat K.G., Mérillon J.M. (2013). Bulbous plants: biotechnology. 1st edition. CRC Press, Boca Raton, USA. [DOI: 10.1201/b16136] [DOI:10.1201/b16136] [PMID] [PMCID]
36. Saeed M.B., Laing M.D. (2023). Biocontrol of maize weevil, Sitophilus zeamais Motschulsky (Coleoptera: Curculionidae), in maize over a six-month storage period. Microorganisms. 11: 1261. [DOI: 10.3390/microorganisms11051261] [DOI:10.3390/microorganisms11051261] [PMID] [PMCID]
37. Salman M.K., Mudalal S. (2022). Quality control and mycotoxin levels in food in the Palestinian market. Food Additives and Contaminants: Part B. 15: 123-128. [DOI: 10.1080/19393210. 2022.2046651] [DOI:10.1080/19393210.2022.2046651] [PMID]
38. Ukwu C.P., Yahaya A., Okere P.C., Aladi N.O., Odoemenam V.U., Obikaonu H.O., Uchegbu M.C., Okoli I.C. (2022). The production, uses, nutritional and anti-nutritional characteristics of cocoyam as a potential feed ingredient in the tropics: a review. Nigerian Journal of Animal Science. 24: 91-111.
39. Zhang Y.-Q., Hui Y., Wang Y., Zhang B., Guo B.-L., Zhang G.-Q., Wei Y.-M. (2020). Effects of drying temperature and relative humidity on quality properties of Chinese dried noodles. Journal of Food Quality. 2020. [DOI: 10.1155/2020/8843974] [DOI:10.1155/2020/8843974]
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