Volume 13, Issue 1 (March 2026)
J. Food Qual. Hazards Control 2026, 13(1): 40-46 |
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Bagheri M, Ghanbarpour R, Amirpoorsaid Z, Mohseni P, Hesam-Arefi M, Jajarmi M. Turkey (Meleagris gallopavo f. domestica) Meat as a Potential Reservoir for Dissemination of Antimicrobial Resistant and Shigatoxigenic Escherichia coli Strains. J. Food Qual. Hazards Control 2026; 13 (1) :40-46
URL: http://jfqhc.ssu.ac.ir/article-1-1247-en.html
URL: http://jfqhc.ssu.ac.ir/article-1-1247-en.html
Department of Food Science and Technology, Bardsir Faculty of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran , ma.bagheri@uk.ac.ir
Abstract: (63 Views)
Background: Enteropathogenic and Shiga toxin-producing Escherichia coli (E. coli) are the two main pathotypes capable of causing serious human infections, especially when resistant to antibiotics.
Methods: In this work, 95 turkey carcasses were swabbed in slaughterhouse over four months (June–September 2023). Antibiotic resistance was evaluated by a disk diffusion method named Kirby–Bauer against nine antimicrobial agents. Three virulence genes including stx1, stx2, eae, three resistance genes including blaTEM, blaSHV, blaCTX-M, and four phylogenetic markers (arpA, chuA, yjaA, TspE4.C2) were screened by Polymerase Chain Reaction (PCR) method. Data were analyzed using Microsoft Excel and SPSS (version 24), with the chi-square test at a significance level of p≤0.05.
Results: Out of 95 carcasses, 72 (75.78%) were E. coli-positive. Among the E. coli isolates, 63.89% were resistant to chloramphenicol, 59.72% to nalidixic acid, and 56.94% to florfenicol. One isolate, classified as extended-spectrum beta-lactamase positive, belonged to phylogroup D and showed simultaneous resistance to four antibiotics without harboring the resistance genes studied. Overall, 52.7% of the E. coli isolates were recognized as Multi-Drug Resistant (MDR). Profiles of resistance genes included blaTEM (23.61%), blaTEM/blaSHV (2.77%), and blaCTX-M (1.38%). Virulence genes were detected in six isolates: stx1 (4.17%), stx2 (1.39%), and eae (2.77%). Phylogenetic analysis revealed five groups: A (19.44%), B1 (36.11%), C (5.55%), D (13.8%), and E (15.27%), while 9.72% remained unclassified.
Conclusion: The occurrence of E. coli isolates harboring virulence and antibiotic-resistance genes in turkey carcasses underscores serious public health risks.
The significant frequency of Multi-Drug Resistant (MDR) E. coli isolates highlights the need for improved monitoring and control measures throughout the food chain.
DOI: 10.18502/jfqhc.13.1.21379
Methods: In this work, 95 turkey carcasses were swabbed in slaughterhouse over four months (June–September 2023). Antibiotic resistance was evaluated by a disk diffusion method named Kirby–Bauer against nine antimicrobial agents. Three virulence genes including stx1, stx2, eae, three resistance genes including blaTEM, blaSHV, blaCTX-M, and four phylogenetic markers (arpA, chuA, yjaA, TspE4.C2) were screened by Polymerase Chain Reaction (PCR) method. Data were analyzed using Microsoft Excel and SPSS (version 24), with the chi-square test at a significance level of p≤0.05.
Results: Out of 95 carcasses, 72 (75.78%) were E. coli-positive. Among the E. coli isolates, 63.89% were resistant to chloramphenicol, 59.72% to nalidixic acid, and 56.94% to florfenicol. One isolate, classified as extended-spectrum beta-lactamase positive, belonged to phylogroup D and showed simultaneous resistance to four antibiotics without harboring the resistance genes studied. Overall, 52.7% of the E. coli isolates were recognized as Multi-Drug Resistant (MDR). Profiles of resistance genes included blaTEM (23.61%), blaTEM/blaSHV (2.77%), and blaCTX-M (1.38%). Virulence genes were detected in six isolates: stx1 (4.17%), stx2 (1.39%), and eae (2.77%). Phylogenetic analysis revealed five groups: A (19.44%), B1 (36.11%), C (5.55%), D (13.8%), and E (15.27%), while 9.72% remained unclassified.
Conclusion: The occurrence of E. coli isolates harboring virulence and antibiotic-resistance genes in turkey carcasses underscores serious public health risks.
The significant frequency of Multi-Drug Resistant (MDR) E. coli isolates highlights the need for improved monitoring and control measures throughout the food chain.
DOI: 10.18502/jfqhc.13.1.21379
Keywords: Shiga-Toxigenic Escherichia coli, Beta-Lactamase, Antibiotic Resistance, Phylogeny, Turkeys, Abattoirs
Type of Study: Original article |
Subject:
Special
Received: 24/07/17 | Accepted: 25/09/15 | Published: 26/03/20
Received: 24/07/17 | Accepted: 25/09/15 | Published: 26/03/20
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