Volume 5, Issue 2 (June 2018)
J. Food Qual. Hazards Control 2018, 5(2): 72-76 |
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Soltan Dallal M, Mazaheri Nezhad Fard R, Sharifi-Yazdi M. Prevalence of sea, seb, tsst, and mecA Genes in Staphylococcus aureus Isolated from Shrimps Sold in Seafood Retailers in Tehran, Iran. J. Food Qual. Hazards Control 2018; 5 (2) :72-76
URL: http://jfqhc.ssu.ac.ir/article-1-437-en.html
URL: http://jfqhc.ssu.ac.ir/article-1-437-en.html
Zoonosis Research Centre, Tehran University of Medical Sciences, P.O. Box: 14155-6446 Tehran, Iran , mksharifiyazdi@gmail.com
Abstract: (4642 Views)
Background: Staphylococcus aureus, a Gram-positive bacterium, is the most prevalent food-borne pathogen in most regions of the world. The current study was carried out with the aim of S. aureus isolation from shrimps sold in Tehran, Iran. Furthermore, the genes of mecA as indicator of methicillin-resistant S. aureus, sea, seb, and tsst encoding enterotoxins were studied in the S. aureus isolates.
Methods: Totally, 150 fresh and 150 frozen shrimp samples were collected from seafood retailers in Tehran. Isolation of S. aureus from the samples was carried out using conventional methods. The target genes were identified using polymerase chain reaction technique. Data were statistically analyzed using SPSS v. 11.5 software.
Results: Out of 150 fresh and 150 frozen samples, 84 samples (28%) were contaminated with S. aureus. The contamination rate in fresh samples (22%) was significantly (p<0.05) lower than in frozen samples (34%). Totally, prevalence rates of sea, seb, tsst, and mecA genes in the isolates were 39.3, 15.5, 4.8, and 28.6%, respectively showing significant (p<0.05) differences.
Conclusion: High prevalence rates of enterotoxigenic and also antibiotic resistance genes in S. aureus isolated from shrimp samples in the current study highlighted worries about risk of staphylococcal food poisoning in Iranian shrimp consumers.
DOI: 10.29252/jfqhc.5.2.7
Methods: Totally, 150 fresh and 150 frozen shrimp samples were collected from seafood retailers in Tehran. Isolation of S. aureus from the samples was carried out using conventional methods. The target genes were identified using polymerase chain reaction technique. Data were statistically analyzed using SPSS v. 11.5 software.
Results: Out of 150 fresh and 150 frozen samples, 84 samples (28%) were contaminated with S. aureus. The contamination rate in fresh samples (22%) was significantly (p<0.05) lower than in frozen samples (34%). Totally, prevalence rates of sea, seb, tsst, and mecA genes in the isolates were 39.3, 15.5, 4.8, and 28.6%, respectively showing significant (p<0.05) differences.
Conclusion: High prevalence rates of enterotoxigenic and also antibiotic resistance genes in S. aureus isolated from shrimp samples in the current study highlighted worries about risk of staphylococcal food poisoning in Iranian shrimp consumers.
DOI: 10.29252/jfqhc.5.2.7
Type of Study: Original article |
Subject:
Special
Received: 17/08/04 | Accepted: 17/12/25 | Published: 18/06/23
Received: 17/08/04 | Accepted: 17/12/25 | Published: 18/06/23
References
1. Ahani N., Alipour-eskandandani M. (2014). Detection of enterotoxigenic Staphylococcus aureus in Schizothorax zarudnyi using PCR method. Zahedan Journal of Research in Medical Sciences. 16: 29-31.
2. Aung K.T., Hsu L.Y., Koh T.H., Hapuarachchi H.C., Chau M.L., Gutierrez R.A., Ng L.C. (2017). Prevalence of methicillin-resistant Staphylococcus aureus (MRSA) in retail food in Singapore. Antimicrobial Resistance and Infection Control. 6: 94. [DOI:10.1186/s13756-017-0255-3]
3. Aydin A., Sudagidan M., Muratoglu K. (2011). Prevalence of staphylococcal enterotoxins, toxin genes and genetic relatedness of foodborne Staphylococcus aureus strains isolated in the Marmara region of Turkey. International Journal of Food Microbiology. 148: 99-106. [DOI:10.1016/j.ijfoodmicro.2011.05.007]
4. Betley M.J., Mekalanos J.J. (1988). Nucleotide sequence of the type A staphylococcal enterotoxin gene. Journal of Bacteriology. 170: 34-41. [DOI:10.1128/jb.170.1.34-41.1988]
5. Di Giannatale E., Prencipe V., Tonelli A., Marfoglia C., Migliorati G. (2011). Characterization of Staphylococcus aureus strains isolated from food for human consumption. Veterinaria Italiana. 47: 165-173.
6. Fueyo J.M., Mendoza M.C., Rodicio M.R., Martin M.C. (2005). Cytotoxin and pyrogenic toxin superantigen gene profiles of Staphylococcus aureus associated with subclinical mastitis in dairy cows and relationships with macrorestriction genomic profiles. Journal of Clinical Microbiology. 43: 1278-1284. [DOI:10.1128/JCM.43.3.1278-1284.2005]
7. Hammad A.M., Watanabe W., Fujii T., Shimamoto T. (2012).Occurrence and characteristics of methicillin-resistant and susceptible Staphylococcus aureus and methicillin-resistant coagulase-negative staphylococci from Japanese retail ready-to-eat raw fish. International Journal of Food Microbiology. 156: 286-289. [DOI:10.1016/j.ijfoodmicro.2012.03.022]
8. Hennekinne J.A., De Buyser M.L., Dragacci S. (2011). Staphylococcus aureus and its food poisoning toxins: characterization and outbreak investigation. FEMS Microbiology Reviews. 36: 815-836. [DOI:10.1111/j.1574-6976.2011.00311.x]
9. Institute of Standards and Industrial Research of Iran (ISIRI). (2006). Microbiology of food and animal feeding stuffs-Horizontal method for the enumeration of positive staphylococci coagulase (Staphylococcus aureus and other species). National Standard No. 6806-3. URL: http://standard.isiri.gov. ir/StandardView.aspx?Id=3618. Accessed 7 February 2017.
10. Institute of Standards and Industrial Research of Iran (ISIRI). (2007). Microbiology of food and animal feeding stuffs -preparation of test samples, initial suspension and decimal dilutions for microbiological examination. Part 1: General rules for the preparation of initial suspension and decimal dilutions. National Standard No. 8923-1. URL: http://standard.isiri.gov. ir/StandardView.aspx?Id=8386. Accessed 7 February 2017.
11. Institute of Standards and Industrial Research of Iran (ISIRI). (2014). Microbiology of food and animal feeding stuffs-horizontal method for the enumeration of microorganisms-colony count technique at 30 °C. National Standard No. 5272-1. URL: http://standard.isiri.gov.ir/StandardView.aspx?Id= 43263. Accessed 7 February 2017.
12. Jones C.L., Khan S.A. (1986). Nucleotide sequence of the enterotoxin B gene from Staphylococcus aureus. Journal of Bacteriology. 166: 29-33. [DOI:10.1128/jb.166.1.29-33.1986]
13. Kim N.H., Yun A.R., Rhee M.S. (2011). Prevalence and classification of toxigenic Staphylococcus aureus isolated from refrigerated ready-to-eat foods (sushi, kimbab and California rolls) in Korea. Journal of Applied Microbiology. 111: 1456-1464. [DOI:10.1111/j.1365-2672.2011.05168.x]
14. Krajden M., Minor J.M., Rifkin O., Comanor L. (1999). Effect of multiple freeze-thaw cycles on hepatitis B virus DNA and hepatitis C virus RNA quantification as measured with branched-DNA technology. Journal of Clinical Microbiology. 37: 1683-1686.
15. Mazaheri Nezhad Fard R., Heuzenroeder M.W., Barton M.D. (2011). Antimicrobial and heavy metal resistance in commensal enterococci isolated from pigs. Veterinary Microbiology. 148: 276-282. [DOI:10.1016/j.vetmic.2010.09.002]
16. Mohamed Hatha A.A., Maqbool T.K., Suresh Kumar S. (2003). Microbial quality of shrimp products of export trade produced from aquacultured shrimp. International Journal of Food Microbiology. 82: 213-221. [DOI:10.1016/S0168-1605(02)00306-9]
17. Mus T.E., Cetinkaya F., Celik U. (2014). Occurrence of Vibrio, Salmonella and Staphylococcus aureus in retail fresh fish, mussel and shrimp. Acta Veterinaria Brno. 83: 75-78. [DOI:10.2754/avb201483020075]
18. Noor R., Hasan M.F., Rahman M.M. (2014). Molecular characterization of the virulent microorganisms along with their drug-resistance traits associated with the export quality frozen shrimps in Bangladesh. Springer Plus. 3: 469. [DOI:10.1186/2193-1801-3-469]
19. Norhana M.W., Poole S.E., Deeth H.C., Dykes G.A. (2010). Prevalence, persistence and control of Salmonella and Listeria in shrimp and shrimp products: a review. Food Control. 21: 343-361. [DOI:10.1016/j.foodcont.2009.06.020]
20. Normanno G., Corrente M., La Salandra G., Dambrosio A., Quaglia N.C., Parisi A., Greco G., Bellacicco A.L., Virgilio S., Celano G.V. (2007). Methicillin-resistant Staphylococcus aureus (MRSA) in foods of animal origin product in Italy. International Journal of Food Microbiology. 117: 219-222. [DOI:10.1016/j.ijfoodmicro.2007.04.006]
21. Nychas G.J.E., Skandamis P.N., Tassou C.C., Koutsoumanis K.P. (2008). Meat spoilage during distribution. Meat Science. 78: 77-89. [DOI:10.1016/j.meatsci.2007.06.020]
22. Schaudien D., Baumgartner W., Herden C. (2007). High preservation of DNA standards diluted in 50% glycerol. Diagnostic Molecular Pathology. 16: 153-157. [DOI:10.1097/PDM.0b013e31803c558a]
23. Simon S.S., Sanjeev S. (2007). Prevalence of enterotoxigenic Staphylococcus aureus in fishery products and fish processing factory workers. Food Control. 18: 1565-1568. [DOI:10.1016/j.foodcont.2006.12.007]
24. Soltan Dallal M.M., Foroushani A.R., Sharifi-Yazdi S., Sharifi-Yazdi M.K., Arfatahery N. (2015). Prevalence of Staphylococcus aureus in shrimps in Tehran during 2013. Journal of Medical Bacteriology. 4: 42-46.
25. Soltan Dallal M.M., Salehipour Z., Eshraghi S., Fallah Mehrabadi J., Bakhtiari R. (2010a). Occurrence and molecular characterization of Staphylococcus aureus strains isolated from meat and dairy products by PCR-RFLP. Annals of Microbiology. 60: 189-196. [DOI:10.1007/s13213-010-0025-4]
26. Soltan Dallal M.M., Salehipour Z., Fallah Mehrabadi J. (2010b). Molecular epidemiology of Staphylococcus aureus in food samples based on the protein A gene polymorphic region DNA sequence. Canadian Journal of Microbiology. 56: 18-21. [DOI:10.1139/W09-111]
27. Su Y.C., Lee Wong A.C. (1995). Identification and purification of a new staphylococcal enterotoxin, H. Journal of Food Protection. 61: 1438-1443.
28. Wongboot W., Chomvarin C., Namwat W. (2015). Phenotypic and genotypic detection of enterotoxins, toxic shock syndrome toxin-1 and of methicillin resistance in Staphylococcus aureus isolated from retail ready-to-eat foods in Northeastern Thailand. Southeast Asian Journal of Tropical Medicine and Public Health. 46: 97-104.
29. Zarei M., Maktabi S., Ghorbanpour M. (2012). Prevalence of Listeria monocytogenes, Vibrio parahaemolyticus, Staphylococcus aureus, and Salmonella spp. in seafood products using multiplex polymerase chain reaction. Foodborne Pathogens and Disease. 9: 108-112. [DOI:10.1089/fpd.2011.0989]
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