Volume 6, Issue 3 (September 2019)
J. Food Qual. Hazards Control 2019, 6(3): 109-114 |
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Sayadnouri S, Soltan Dallal M, Akbarzadeh S, Mazaheri Nezhad Fard R. Evaluation of Class 1 and 2 Integrons and Antibiotic Resistance Pattern in Salmonella enterica Isolated from Diarrheal Food-Borne Outbreaks in Iran. J. Food Qual. Hazards Control 2019; 6 (3) :109-114
URL: http://jfqhc.ssu.ac.ir/article-1-578-en.html
URL: http://jfqhc.ssu.ac.ir/article-1-578-en.html
Food Microbiology Research Center/Division of Food Microbiology, Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran , soltanda@sina.tums.ac.ir
Abstract: (2587 Views)
Background: Salmonella spp. are major causes of food-borne disease and have been identified among many diarrheal outbreaks. The major aim of the current investigation was to evaluate the class 1 and 2 integrons and antibiotic resistance pattern in Salmonella enterica isolated from diarrheal food-borne outbreaks in Iran.
Methods: This study was carried out on 115 diarrheal feces samples obtained from food-borne outbreak in 2016 in Iran. Antimicrobial resistance patterns of 27 isolated S. enterica seovars and presence of class 1 and class 2 integrons in the serovars were investigated using conventional and molecular methods. Results were statistically analyzed using SPSS software v. 21 and Chi-Square test.
Results: Overall, 27 S. enterica were characterized as 14 S. Paratyphi C, 7 S. Enteritidis, 5 S. Paratyphi D, and 1 S. Paratyphi A serovars. Results of molecular assay showed that class 1 integron presented in all and class 2 integron in three strains. All isolates with class 2 integron genes were resistant to almost all the antimicrobials.
Conclusion: Most studied Salmonella strains from diarrheal food-borne outbreak of Iran in 2016 were multiple resistant to the highlighted antimicrobials. Knowledge about risk factor involving the salmonellosis and their control measures could help the national authorities to prevent the outbreaks. Further comprehensive studies with larger sample sizes are necessary to acquire more data about risk factors of multiple resistant Salmonella outbreaks in the country.
DOI: 10.18502/jfqhc.6.3.1384
Methods: This study was carried out on 115 diarrheal feces samples obtained from food-borne outbreak in 2016 in Iran. Antimicrobial resistance patterns of 27 isolated S. enterica seovars and presence of class 1 and class 2 integrons in the serovars were investigated using conventional and molecular methods. Results were statistically analyzed using SPSS software v. 21 and Chi-Square test.
Results: Overall, 27 S. enterica were characterized as 14 S. Paratyphi C, 7 S. Enteritidis, 5 S. Paratyphi D, and 1 S. Paratyphi A serovars. Results of molecular assay showed that class 1 integron presented in all and class 2 integron in three strains. All isolates with class 2 integron genes were resistant to almost all the antimicrobials.
Conclusion: Most studied Salmonella strains from diarrheal food-borne outbreak of Iran in 2016 were multiple resistant to the highlighted antimicrobials. Knowledge about risk factor involving the salmonellosis and their control measures could help the national authorities to prevent the outbreaks. Further comprehensive studies with larger sample sizes are necessary to acquire more data about risk factors of multiple resistant Salmonella outbreaks in the country.
DOI: 10.18502/jfqhc.6.3.1384
Type of Study: Original article |
Subject:
Special
Received: 18/06/24 | Accepted: 19/02/07 | Published: 19/09/03
Received: 18/06/24 | Accepted: 19/02/07 | Published: 19/09/03
References
1. Ahmed A.M., Nakano H., Shimamoto T. (2005). Molecular characterization of integrons in non-typhoid Salmonella serovars isolated in Japan: description of an unusual class 2 integron. The Journal of Antimicrobial Chemotherapy. 55: 371-374. [DOI: 10.1093/jac/dkh534] [DOI:10.1093/jac/dkh534] [PMID]
2. Alizadeh-Hesar M., Bakhshi B., Najar-Peerayeh S. (2014). Molecular diagnosis of Salmonella enterica and Shigella spp. in stool sample of children with diarrhea in Tehran. International Journal of Enteric Pathogens. 2: e17002. [DOI: 10.17795/ ijep17002] [DOI:10.17795/ijep17002]
3. Cabrera R., Marco F., Vila J., Ruiz J., Gascon J. (2006). Class 1 integrons in Salmonella strains causing traveler's diarrhea. Antimicrobial Agents and Chemotherapy. 50: 1612-1613. [DOI: 10.1128/AAC.50.4.1612-1613.2006] [DOI:10.1128/AAC.50.4.1612-1613.2006] [PMID] [PMCID]
4. Caleja C., de Toro M., Goncalves A., Themudo P., Vieira-Pinto M., Monteiro D., Rodrigues J., Saenz Y., Carvalho C., Igrejas G., Torres C., Poeta P. (2011). Antimicrobial resistance and class I integrons in Salmonella enterica isolates from wild boars and Bisaro pigs. International Microbiology. 14: 19-24. [DOI: 10.2436/20.1501.01.131]
5. Clinical and Laboratory Standards Institute (CLSI). (2015). Performance standards for antimicrobial disk susceptibility tests. 12th edition. CLSI Document M02-A12. Wayne, USA.
6. Cummings K.J., Perkins G.A., Khatibzadeh S.M., Warnick L.D., Altier C. (2013). Antimicrobial resistance trends among Salmonella isolates obtained from dairy cattle in the northeastern United States, 2004-2011. Foodborne Pathogens and Disease. 10: 353-361. DOI: [10.1089/fpd.2012.1285] [DOI:10.1089/fpd.2012.1285]
7. Edrington T.S., Schultz C.L., Bischoff K.M., Callaway T.R., Looper M.L., Genovese K.J., Jung Y.S., McReynolds J.L., Anderson R.C., Nisbet D.J. (2004). Antimicrobial resistance and serotype prevalence of Salmonella isolated from dairy cattle in the Southwestern United States. Microbial Drug Resistance. 10: 51-56. [DOI: 10.1089/107662904323047808] [DOI:10.1089/107662904323047808] [PMID]
8. El-Demerdash A.S., Aggour M.G., El-Azzouny M.M., Abou-Khadra S.H. (2018). Molecular analysis of integron gene cassette arrays associated multi-drug resistant Enterobacteriaceae isolates from poultry. Cellular and Molecular Biology. 64: 149-156. [DOI: 10.14715/cmb/2018.64.5.25] [DOI:10.14715/cmb/2018.64.5.25] [PMID]
9. Eshaghi Zadeh S.H., Fahimi H., Fardsanei F., Soltan Dallal M.M. (2019). Antimicrobial resistance and presence of class 1 integrons among different serotypes of Salmonella spp. recovered from children with diarrhea in Tehran, Iran. Infectious Disorders-Drug Targets. [DOI: 10.2174/ 1871526519666190130171020] [DOI:10.2174/1871526519666190130171020] [PMID]
10. Fardsanei F., Soltan Dallal M.M., Douraghi M., Memariani H., Bakhshi B., Zahraei Salehi T., Nikkhahi F. (2018). Antimicrobial resistance, virulence genes and genetic relatedness of Salmonella enterica serotype Enteritidis isolates recovered from human gastroenteritis in Tehran, Iran. Journal of Global Antimicrobial Resistance. 12: 220-226. [DOI: 10.1016/j.jgar. 2017.10.005] [DOI:10.1016/j.jgar.2017.10.005] [PMID]
11. Fardsanei F., Soltan Dallal M.M., Douraghi M., Zahraei Salehi T., Mahmoodi M., Memariani H., Nikkhahi F. (2017). Genetic diversity and virulence genes of Salmonella enterica subspecies enterica serotype Enteritidis isolated from meats and eggs. Microbial Pathogenesis. 107: 451-456. [DOI: 10.1016/j. micpath.2017.04.026] [DOI:10.1016/j.micpath.2017.04.026] [PMID]
12. Ferrari R.G., Rosario D.K.A., Cunha-Neto A., Mano S.B., Figueiredo E.E.S., Conte-Junior C.A. (2019). Worldwide epidemiology of Salmonella serovars in animal-based foods: a meta-analysis. Applied and Environmental Microbiology. 85: e00591-19. [DOI: 10.1128/AEM.00591-19] [DOI:10.1128/AEM.00591-19] [PMID]
13. Firoozeh F., Shahcheraghi F., Zahraei Salehi T., Karimi V., Aslani M.M. (2011). Antimicrobial resistance profile and presence of class I integrongs among Salmonella enterica serovars isolated from human clinical specimens in Tehran, Iran. Iranian Journal of Microbiology. 3: 112-117.
14. Gassama-Sow A., Aidara-Kane A., Raked N., Denis F., Ploy M.C. (2004). Integrons in Salmonella Keurmassar, Senegal. Emerging Infectious Diseases. 10: 1339-1341. [DOI: 10.3201/eid1007.030666] [DOI:10.3201/eid1007.030666] [PMID] [PMCID]
15. Jackson B.R., Griffin P.M., Cole D., Walsh K.A., Chai S.J. (2013). Outbreak-associated Salmonella enterica serotypes and food commodities, United States, 1998-2008. Emerging Infectious Diseases. 19: 1239-1244. [DOI: 10.3201/eid1908.121511] [DOI:10.3201/eid1908.121511] [PMID] [PMCID]
16. Liang Z., Ke B., Deng X., Liang J., Ran L., Lu L., He D., Huang Q., Ke C., Li Z., Yu H., Klena J.D., et al. (2015). Serotypes, seasonal trends, and antibiotic resistance of non-typhoidal Salmonella from human patients in Guangdong Province, China, 2009-2012. BMC Infectious Diseases. 15: 53. [DOI: 10.1186/s12879-015-0784-4] [DOI:10.1186/s12879-015-0784-4] [PMID] [PMCID]
17. McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., et al. (2001). Complete genome sequence of Salmonella enterica serovar Typhimurium LT2. Nature. 413: 852-856. [DOI: 10.1038/35101614] [DOI:10.1038/35101614] [PMID]
18. Ploy M.C., Chainier D., Thi N.H.T., Poilane I., Cruaud P., Denis F., Collignon A., Lambert T. (2003). Integron-associated antibiotic resistance in Salmonella enterica serovar Typhi from Asia. Antimicrobial Agents and Chemotherapy. 47: 1427-1429. [DOI: 10.1128/AAC.47.4.1427-1429.2003] [DOI:10.1128/AAC.47.4.1427-1429.2003] [PMID] [PMCID]
19. Porwollik S., Boyd E.F., Choy C., Cheng P., Florea L., Proctor E., McClelland M. (2004). Characterization of Salmonella enterica subspecies I genovars by use of microarrays. Journal of Bacteriology. 186: 5883-5898. [DOI: 10.1128/JB.186.17. 5883-5898.2004] [DOI:10.1128/JB.186.17.5883-5898.2004] [PMID] [PMCID]
20. Rahmani M., Peighambari S.M., Svendsen C.A., Cavaco L.M., Agersø Y., Hendriksen R.S. (2013). Molecular clonality and antimicrobial resistance in Salmonella enterica serovars Enteritidis and Infantis from broilers in three Northern regions of Iran. BMC Veterinary Research. 9: 66. [DOI: 10.1186/ 1746-6148-9-66] [DOI:10.1186/1746-6148-9-66] [PMID] [PMCID]
21. Ratajczak M., Laroche E., Berthe T., Clermont O., Pawlak B., Denamur E., Petit F. (2010). Influence of hydrological conditions on the Escherichia coli population structure in the water of a creek on a rural watershed. BMC Microbiology. 10: 222. [DOI: 10.1186/1471-2180-10-222] [DOI:10.1186/1471-2180-10-222] [PMID] [PMCID]
22. Sousa M.A.B., Mendes E.N., Penna F.J., Peret-Filho L.A., Magalhaes P.P. (2013). Acute diarrhea associated with Salmonella enterica in Belo Horizonte-MG: prevalence and characterization of isolates. Jornal Brasileiro de Patologia e Medicina Laboratorial. 49: 34-38. [DOI: 10.1590/S1676-24442013000100005] [DOI:10.1590/S1676-24442013000100005]
23. Vo A.T., Van Duijkeren E., Gaastra W., Fluit A.C. (2010). Antimicrobial resistance, class 1 integrons, and genomic island 1 in Salmonella isolates from Vietnam. PloS One. 5: e9440. [DOI: 10.1371/journal.pone.0009440] [DOI:10.1371/journal.pone.0009440] [PMID] [PMCID]
24. World Health Organization (WHO). (2017). Diarrhoeal disease fact sheet. WHO library. Geneva, Switzerland. 2 May 2017.
25. Wright A.P., Richardson L., Mahon B.E., Rothenberg R., Cole D.J. (2016). The rise and decline in Salmonella enterica serovar Enteritidis outbreaks attributed to egg-containing foods in the United States, 1973-2009. Epidemiology and Infection. 144: 810-819. [DOI: 10.1017/S0950268815001867] [DOI:10.1017/S0950268815001867] [PMID]
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