Volume 7, Issue 2 (June 2020)                   J. Food Qual. Hazards Control 2020, 7(2): 94-107 | Back to browse issues page


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Ercole C, Centi V, Pellegrini M, Marotta F, Del Gallo M. Effect of Ripening Time and Seasonal Changes on Microbial and Physicochemical Properties of Inland Pecorino Abruzzese Cheese. J. Food Qual. Hazards Control 2020; 7 (2) :94-107
URL: http://jfqhc.ssu.ac.ir/article-1-667-en.html
Department of Life, Health and Environmental Sciences, University of L’Aquila, Coppito, 67100 L’Aquila, Italy , claudia.ercole@univaq.it
Abstract:   (2203 Views)
Background: Hand-made cheeses are usually prepared following dissimilar procedures which influence the quality and the organoleptic properties of the products. Objective of the present study was to evaluate how manufacturing season and ripening time affect hand-made Pecorino Abruzzese cheese.
Methods: Microbiological and physicochemical characteristics were investigated on raw milk cheeses produced in spring and autumn sampled at different ripening times (20, 60, 120, 210, and 300 days). Statistical analysis was done using SPSS software version 21.
Results: Spring Marzolino cheeses showed better quality than those produced in autumn, with higher contents of protein, moisture, and Water-Soluble Nitrogen/Total Nitrogen (WSN/TN); and lower content of fat and salt. Besides, Marzolino samples exhibited an extensive αS1-casein proteolysis, slight hydrolysis of β-casein, low levels of γ-casein, and the occurrence of heterogeneous mixtures of proteolytic products as well as more complex microbial populations. At 20 days of ripening, all spring-cheese microbial groups presented in a remarkably high number than that presented in autumn, whereas enterococci populations were significantly higher in autumn cheeses than in spring ones (7 and 6 log Colony Forming Unit/g for autumn and spring, respectively). Ripening demonstrated a positive effect, in both productions, by increasing the concentration of the physicochemical parameters and a decrease of microbial populations of 1-3 log units.
Conclusion: Marzolino cheeses, manufactured in springtime, had better quality profile than those manufactured in autumn which this finding could be utilized to set up marketing strategies.

DOI: 10.18502/jfqhc.7.2.2889
Full-Text [PDF 990 kb]   (801 Downloads)    
Type of Study: Original article | Subject: Special
Received: 20/01/11 | Accepted: 20/03/29 | Published: 20/06/18

References
1. Addis M., Fiori M., Riu G., Pes M., Salvatore E., Pirisi A. (2015). Physico-chemical characteristics and acidic profile of PDO Pecorino Romano cheese: seasonal variation. Small Ruminant Research. 126: 73-79. [DOI: 10.1016/j.smallrumres.2015.03. 014] [DOI:10.1016/j.smallrumres.2015.03.014]
2. Aminifar M., Hamedi M., Emam-Djomeh Z., Mehdinia A. (2014). Investigation on proteolysis and formation of volatile compounds of Lighvan cheese during ripening. Journal of Food Science and Technology. 51: 2454‐2462. [DOI:10.1007/ s13197-012-0755-3] [DOI:10.1007/s13197-012-0755-3] [PMID] [PMCID]
3. Andrews A.T. (1983). Proteinases in normal bovine milk and their action on caseins. Journal of Dairy Research. 50: 45-55. [DOI: 10.1017/s0022029900032519] [DOI:10.1017/S0022029900032519] [PMID]
4. Aquilanti L., Dell'Aquila L., Zannini E., Zocchetti A., Clementi F. (2006). Resident lactic acid bacteria in raw milk Canestrato Pugliese cheese. Letters in Applied Microbiology. 43: 161-167. [DOI: 10.1111/j.1472-765X.2006.01935.x] [DOI:10.1111/j.1472-765X.2006.01935.x] [PMID]
5. Association of Official Analytical Chemists (AOAC). (1990). Official methods of analysis of the association of official analytical chemists. 15th edition. Washington, DC.
6. Association of Official Analytical Chemists (AOAC). (1999). Official methods of analysis of the association of official analytical chemists. 16th edition. Gaithersburg.
7. Barron L.J.R., Fernández de Labastida E., Perea S., Chávarri F., de Vega C., Vicente M.S., Torres M.I., Nájera A.I., Virto M., Santisteban A., Pérez-Elortondo F.J., Albisu M., et al. (2001). Seasonal changes in the composition of bulk raw ewe's milk used for Idiazabal cheese manufacture. International Dairy Journal. 11: 771-778. [DOI: 10.1016/ S0958-6946(01)00120-0] [DOI:10.1016/S0958-6946(01)00120-0]
8. Blakesley R.W., Boezi J.A. (1977). A new staining technique for proteins in polyacrylamide gels using Coomassie Brilliant Blue G250. Analytical Biochemistry. 82: 580-582. [DOI: 10.1016/0003-2697(77)90197-x] [DOI:10.1016/0003-2697(77)90197-X]
9. Castro R.D., Oliveira L.G., Sant'Anna F.M., Luiz L.M.P., Sandes S.H.C., Silva C.I.F., Silva A.M., Nunes A.C., Penna C.F.A.M., Souza M.R. (2016). Lactic acid microbiota identification in water, raw milk, endogenous starter culture, and fresh Minas artisanal cheese from the Campo das Vertentes region of Brazil during the dry and rainy seasons. Journal of Dairy Science. 99: 6086-6096. [DOI: 10.3168/jds.2015-10579] [DOI:10.3168/jds.2015-10579] [PMID]
10. Centi V., Matteucci F., Lepidi A., Del Gallo M., Ercole C. (2017). Microbiological and biochemical aspects of inland Pecorino Abruzzese cheese. Heliyon. 3: e00258. [DOI: 10.1016/j. heliyon.2017.e00258] [DOI:10.1016/j.heliyon.2017.e00258] [PMID] [PMCID]
11. Chilliard Y., Ferlay A., Rouel J., Lamberet G. (2003). A review of nutritional and physiological factors affecting goat milk lipid synthesis and lipolysis. Journal of Dairy Science. 86: 1751-1770. [DOI: 10.3168/jds.S0022-0302(03)73761-8] [DOI:10.3168/jds.S0022-0302(03)73761-8]
12. Collins Y.F., McSweeney P.L.H., Wilkinson M.G. (2003). Lipolysis and free fatty acid catabolism in cheese: a review of current knowledge. International Dairy Journal. 13: 841-866. [DOI: 10.1016/S0958-6946(03)00109-2] [DOI:10.1016/S0958-6946(03)00109-2]
13. De Jong C., Badings H.T. (1990). Determination of free fatty acids in milk and cheese procedures for extraction, clean up, and capillary gas chromatographic analysis. Journal of High Resolution Chromatography. 13: 94-98. [DOI: 10.1002/jhrc. 1240130204] [DOI:10.1002/jhrc.1240130204]
14. Di Cagno R., Banks J., Sheehan L., Fox P.F., Brechany E.Y., Corsetti A., Gobbetti M. (2003). Comparison of the microbiological, compositional, biochemical, volatile profile and sensory characteristics of three Italian PDO ewes' milk cheeses. International Dairy Journal. 13: 961-972. [DOI: 10.1016/ S0958-6946(03)00145-6] [DOI:10.1016/S0958-6946(03)00145-6]
15. Espinosa-Pesqueira D., Hernández-Herrero M.M., Roig-Sagués A.X. (2018). High hydrostatic pressure as a tool to reduce formation of biogenic amines in artisanal Spanish cheeses. Foods. 7: 137. [DOI: 10.3390/foods7090137] [DOI:10.3390/foods7090137] [PMID] [PMCID]
16. Farahani Z., Rasooli I., Owlia P. (2017). Isolation, identification and characterization of indigenous lactic acid bacteria for flavor improvement. International Food Research Journal. 24: 428-436.
17. Fenelon M.A., O'Connor P., Guinee T.P. (2000). The effect of fat content on the microbiology and proteolysis in Cheddar cheese during ripening. Journal of Dairy Science. 83: 2173-2183. [DOI: 10.3168/jds.S0022-0302(00)75100-9] [DOI:10.3168/jds.S0022-0302(00)75100-9]
18. Fernández-Garcia E., Gaya P., Medina M., Nuñez M. (2004). Evolution of the volatile components of raw ewes' milk Castellano cheese: seasonal variation. International Dairy Journal. 14: 39-46. [DOI: 10.1016/S0958-6946(03)00148-1] [DOI:10.1016/S0958-6946(03)00148-1]
19. Gaglio R., Todaro M., Scatassa M.L., Franciosi E., Corona O., Mancuso I., De Gerlando R., Cardamone C., Settanni L. (2019). Transformation of raw ewes' milk applying "Grana" type pressed cheese technology: development of extra-hard "Gran Ovino" cheese. International Journal of Food Microbiology. 307: 108277. [DOI: 10.1016/j.ijfoodmicro. 2019.108277] [DOI:10.1016/j.ijfoodmicro.2019.108277] [PMID]
20. Gaiaschi A., Beretta B., Poiesi C., Conti A., Giuffrida M.G., Galli C.L., Restani P. (2001). Proteolysis of β-casein as a marker of Grana Padano cheese ripening. Journal of Dairy Science. 84: 60-65. [DOI: 10.3168/jds.S0022-0302(01)74452-9] [DOI:10.3168/jds.S0022-0302(01)74452-9]
21. Gobbetti M., Morea M., Baruzzi F., Corbo M.R., Matarante A., Considine T., Di Cagno R., Guinee T., Fox P.F. (2002). Microbiological compositional, biochemical and textural characterisation of Caciocavallo Pugliese cheese during ripening. International Dairy Journal. 12: 511-523. [DOI: 10.1016/S0958-6946(02)00042-0] [DOI:10.1016/S0958-6946(02)00042-0]
22. Hynes E., Bach C., Lamberet G., Ogier J.C., Son O., Delacroix-Buchet A. (2003). Contribution of starter lactococci and adjunct lactobacilli to proteolysis, volatile profiles and sensory characteristics of washed-curd cheese. Lait. 83: 31-43. [DOI: 10.1051/lait:2002048] [DOI:10.1051/lait:2002048]
23. Inácio R.S., Gomes A.M.P., Saraiva J.A. (2020). Serra da Estrela cheese: a review. Journal of Food Processing and Preservation. 44: e14412. [DOI: 10.1111/jfpp.14412] [DOI:10.1111/jfpp.14412]
24. International Dairy Federation (IDF). (1964). Determination of the protein content of processed cheese products. Standard No. 25. International Dairy Federation, Brussels.
25. International Dairy Federation (IDF). (1988). Determination of salt content. Standard No. 12B. International Dairy Federation, Brussels.
26. International Dairy Federation (IDF). (1989). Determination of pH. Standard No. 115a. International Dairy Federation, Brussels.
27. International Dairy Federation (IDF). (1990). Determination of milk fat, protein and lactose content. International Dairy Federation, Brussels.
28. International Dairy Federation (IDF). (1993). Determination of the nitrogen (Kjeldahl method) and calculation of the crude protein content. Standard No. 20B. International Dairy Federation, Brussels.
29. International Organization for Standardization (ISO). (2004). Whey cheese-determination of dry matter. No. 2920.
30. Kafili T., Razavi S.H., Emam-Djomeh Z., Naghavi M.R., Alvarez-Martin P., Mayo B. (2009). Microbial characterization of Iranian traditional Lighvan cheese over manufacturing and ripening via culturing and PCR-DGGE analysis: identification and typing of dominant lactobacilli. European Food Research and Technology. 229: 83-92. [DOI: 10.1007/s00217-009-1028-x] [DOI:10.1007/s00217-009-1028-x]
31. Khattab A.R., Guirguis H.A., Tawfik S.M., Farag M.A. (2019). Cheese ripening: a review on modern technologies towards flavor enhancement, process acceleration and improved quality assessment. Trends in Food Science and Technology. 88: 343-360. [DOI: 10.1016/j.tifs.2019.03.009] [DOI:10.1016/j.tifs.2019.03.009]
32. Martín-Platero A.M., Valdivia E., Maqueda M., Martín-Sánchez I., Martínez-Bueno M. (2008). Polyphasic approach to bacterial dynamics during the ripening of Spanish farmhouse cheese, using culture-dependent and -independent methods. Applied and Environmental Microbiology. 74: 5662-5673. [DOI: 10.1128/AEM.00418-08] [DOI:10.1128/AEM.00418-08] [PMID] [PMCID]
33. McSweeney P.L.H. (2004). Biochemistry of cheese ripening. International Journal of Dairy Technology. 57: 127-144. [DOI: 10.1111/j.1471-0307.2004.00147.x] [DOI:10.1111/j.1471-0307.2004.00147.x]
34. Ogier J.C., Serror P. (2008). Safety assessment of dairy microorganisms - the Enterococcus genus. International Journal of Food Microbiology. 126: 291-301. [DOI: 10.1016/j.ijfoodmicro. 2007.08.017] [DOI:10.1016/j.ijfoodmicro.2007.08.017] [PMID]
35. Polychroniadau A. (1988). A simple procedure using trinitrobenzenesulfonic acid for monitoring proteolysis in cheese. Journal of Dairy Research. 55: 585-596. [DOI: 10.1017/S0022029900033379] [DOI:10.1017/S0022029900033379]
36. Poznanski E., Cavazza A., Cappa F., Cocconcelli P.S. (2004). Indigenous raw milk microbiota influences the bacterial development in traditional cheese from alpine natural park. International Journal of Food Microbiology. 92: 141-151. [DOI: 10.1016/j.ijfoodmicro.2003.09.006] [DOI:10.1016/j.ijfoodmicro.2003.09.006] [PMID]
37. Pripp A.H., Shakeel-Ur-Rehman, McSweeney P.L.H., Fox P.F. (1999). Multivariate statistical analysis of peptide profiles and free amino acids to evaluate effects of single-strain starters on proteolysis in miniature Cheddar-type cheeses. International Dairy Journal. 9: 473-479. [DOI: 10.1016/S0958-6946(99) 00116-8] [DOI:10.1016/S0958-6946(99)00116-8]
38. Quigley L., O'Sullivan O., Beresford T.P., Ross R.P., Fitzgerald G.F., Cotter P.D. (2011). Molecular approaches to analysing the microbial composition of raw milk and raw milk cheese. International Journal of Food Microbiology. 150: 81-94. [DOI: 10.1016/j.ijfoodmicro.2011.08.001] [DOI:10.1016/j.ijfoodmicro.2011.08.001] [PMID]
39. Randazzo C.L., Pitino I., Ribbera A., Caggia C. (2010). Pecorino Crotonese cheese: study of bacterial population and flavor compounds. Food Microbiology. 27: 363-374. [DOI: 10. 1016/j.fm.2009.11.010] [DOI:10.1016/j.fm.2009.11.010]
40. Renes E., Ladero V., Tornadijo M.E., Fresno J.M. (2019). Production of sheep milk cheese with high γ-aminobutyric acid and ornithine concentration and with reduced biogenic amines level using autochthonous lactic acid bacteria strains. Food Microbiology. 78: 1-10. [DOI: 10.1016/j.fm.2018.09.003] [DOI:10.1016/j.fm.2018.09.003] [PMID]
41. Schirone M., Tofalo R., Mazzone G., Corsetti A., Suzzi G. (2011). Biogenic amine content and microbiological profile of Pecorino di Farindola cheese. Food Microbiology. 28: 128-136. [DOI: 10.1016/j.fm.2010.09.005] [DOI:10.1016/j.fm.2010.09.005] [PMID]
42. Schirone M., Tofalo R., Visciano P., Corsetti A., Suzzi G. (2012). Biogenic amines in Italian Pecorino cheese. Frontiers in Microbiology. 3: 171. [DOI: 10.3389/fmicb.2012.00171] [DOI:10.3389/fmicb.2012.00171]
43. Sevi A., Albenzio M., Marino R., Santillo A., Muscio A. (2004). Effects of lambing season and stage of lactation on ewe milk quality. Small Ruminant Research. 51: 251-259. [DOI: 10.1016/S0921-4488(03)00196-2] [DOI:10.1016/S0921-4488(03)00196-2]
44. Sevi A., Annicchiarico G., Albenzio M., Taibi L., Muscio A., Dell'Aquila S. (2001). Effects of solar radiation and feeding time on behavior, immune response and production of lactating ewes under high ambient temperature. Journal of Dairy Science. 84: 629-640. [DOI: 10.3168/jds.S0022-0302(01) 74518-3] [DOI:10.3168/jds.S0022-0302(01)74518-3]
45. Shakeel-Ur-Rehman, McSweeney P.L.H., Fox P.F. (1998). Protocol for the manufacture of miniature cheeses. Lait. 78: 607-620. [DOI: 10.1051/lait:1998654] [DOI:10.1051/lait:1998654]
46. Sousa M.J., McSweeney P.L.H. (2001). Studies on the ripening of Cooleeney, an Irish farmhouse Camembert-type cheese. Irish Journal of Agricultural and Food Research. 40: 83-95.
47. Todaro M., Bonanno A., Scatassa M.L. (2014). The quality of Valle del Belice sheep's milk and cheese produced in the hot sum- mer season in Sicily. Dairy Science and Technology. 94: 225-239. [DOI: 10.1007/s13594-013-0155-0] [DOI:10.1007/s13594-013-0155-0]

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