Volume 9, Issue 4 (December 2022)
J. Food Qual. Hazards Control 2022, 9(4): 199-214 |
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Shehata M, Badr A, Abd El-Aziz N, Abd-Rabou H, El-Sohaimy S. Optimization, Partial Purification, and Characterization of Bioactive Peptides of Lactobacillus paracasei Isolated from Traditional Egyptian Cheese. J. Food Qual. Hazards Control 2022; 9 (4) :199-214
URL: http://jfqhc.ssu.ac.ir/article-1-1027-en.html
URL: http://jfqhc.ssu.ac.ir/article-1-1027-en.html
Department of Food Technology, Arid Lands Cultivation Research Institute, City of Scientific Research and Technological Applications (SRTA-City), New Borg El-Arab 21,934, Egypt, Department of Technology and Organization of Public Catering, Institute of Sport, Tourism and Service, South Ural State University, Chelyabinsk, Russia , elsohaimys@gmail.com
Abstract: (693 Views)
Background: Bacteriocins are small peptides which are ribosomally synthesized and have been shown to have wide range of antimicrobial activity. The aim of this study was to optimize the production of L. paracasei MG847589 bacteriocin. Furthermore, the potential antibacterial properties of the novel bacteriocins were characterized and evaluated against Staphylococcus aureus.
Methods: The present study optimized the growth media constituents of Lactobacillus paracasei MG847589 to improve bacteriocin yield by applying One-Factor-at-a-Time (OFAT) and Response Surface Methodology (RSM) methods.
Results: At OFAT, two-fold activity increased against Staphylococcus aureus in the presence of whey (22.5 g/L) as nitrogen source and sucrose (30 g/L) as carbon source. RSM tool was performed with media compounds using design expert 12.0.1.0. Whey (22.5 g/L), sucrose (30 g/L), temperature (30 ºC), and pH (6.5) condition yielded 25,600 AU/ml of bacteriocin against S. aureus. Bacteriocin was stable at pH range of 2.0 to 8.0 for one h and at 60 ºC for 15 min. The produced antimicrobial peptide is a novel bacteriocin with molecular mass of 2,611.122 Da.
Conclusion: Bacteriocin of L. paracasei MG847589 isolated from traditional Egyptian cheese (Kareish) showed great antimicrobial activity and could be applied as food preservative in food manufacturing.
DOI: 10.18502/jfqhc.9.4.11375
Methods: The present study optimized the growth media constituents of Lactobacillus paracasei MG847589 to improve bacteriocin yield by applying One-Factor-at-a-Time (OFAT) and Response Surface Methodology (RSM) methods.
Results: At OFAT, two-fold activity increased against Staphylococcus aureus in the presence of whey (22.5 g/L) as nitrogen source and sucrose (30 g/L) as carbon source. RSM tool was performed with media compounds using design expert 12.0.1.0. Whey (22.5 g/L), sucrose (30 g/L), temperature (30 ºC), and pH (6.5) condition yielded 25,600 AU/ml of bacteriocin against S. aureus. Bacteriocin was stable at pH range of 2.0 to 8.0 for one h and at 60 ºC for 15 min. The produced antimicrobial peptide is a novel bacteriocin with molecular mass of 2,611.122 Da.
Conclusion: Bacteriocin of L. paracasei MG847589 isolated from traditional Egyptian cheese (Kareish) showed great antimicrobial activity and could be applied as food preservative in food manufacturing.
DOI: 10.18502/jfqhc.9.4.11375
Keywords: Bacteriocins, Peptides, Lactobacillales, Lacticaseibacillus paracasei, Anti-Bacterial Agents, Staphylococcus aureus
Type of Study: Original article |
Subject:
Special
Received: 22/03/14 | Accepted: 22/10/23 | Published: 22/12/29
Received: 22/03/14 | Accepted: 22/10/23 | Published: 22/12/29
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