Volume 9, Issue 2 (June 2022)                   J. Food Qual. Hazards Control 2022, 9(2): 98-104 | Back to browse issues page


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Shahrokhi S, Hajimohammadi B, Eslami G, Ehrampoush M, Fallahzadeh M. Occurrence of Cryptosporidium spp. in Traditional Milk and Dairy Products Supplied in Yazd City, Central Iran. J. Food Qual. Hazards Control 2022; 9 (2) :98-104
URL: http://jfqhc.ssu.ac.ir/article-1-1037-en.html
Department of Parasitology and Mycology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran , eslami _g2000@yahoo.com
Abstract:   (415 Views)
Background: Cryptosporidium is one of the most important agents of food-borne diseases with gastrointestinal symptoms such as diarrhea in either livestock or humans. This protozoon can be transmitted to human through consuming contaminated raw milk and dairy products. The present study aimed to detect Cryptosporidium spp. in traditional raw cow milk, cream, and cheese consumed in Yazd city, Central Iran.
Methods: Two hundred traditional (unpasteurized) milk and dairy samples were collected from five different regions of the studied area, including 100 traditional cow milk, 50 cow cheese, and 50 cow cream. DNA extraction was performed. Then, molecular detection was performed using the nested Polymerase Chain Reaction technique. Statistical analysis was performed using SPSS version 23.0.
Results: The findings of this study showed that 11% samples were contaminated with Cryptosporidium spp., including 4.0% (4/100) milk, 18.0% (9/50) cream, and 18.0% (9/50) cheese (p<0.05). Besides, parasite contamination was 8.8, 12.5, 12.8, 8.3, and 12.8% in the Center, North, East, South, and West of the studied region, respectively (p>0.05).
Conclusion: Due to the occurrence of Cryptosporidium spp. in unpasteurized dairy samples in Central Iran, developing and designing control and prevention programs is necessary against this parasite.

DOI: 10.18502/jfqhc.9.2.10646
Full-Text [PDF 390 kb]   (248 Downloads)    
Type of Study: Original article | Subject: Special
Received: 21/10/15 | Accepted: 22/02/28 | Published: 22/06/27

References
1. Alum A., Absar I.M., Asaad H., Rubino J.R., Ijaz M.K. (2014). Impact of environmental conditions on the survival of Cryptosporidium and Giardia on environmental surfaces. Interdisciplinary Perspectives on Infectious Diseases. 2014. [DOI: 10.1155/2014/210385] [DOI:10.1155/2014/210385] [PMID] [PMCID]
2. Badoui L., Dabo G., Lamdini H., Oulad Lahcen A., Sodqi M., Marih L., Chakib A., Soussi M., El Filali K.M. (2014). Intestinal cryptosporidiosis in HIV-infected patients in the department of infectious diseases. BMC Infectious Diseases. 14: P52. [DOI: 10.1186/1471-2334-14-S2-P52] [DOI:10.1186/1471-2334-14-S2-P52] [PMCID]
3. Bakir M.H.H. (2005). The study of epidemiology, experimental, immumity and transmission for Cryptosporidium in Nineveh Governorate Ph.D. Thesis, College of Veterinary Medicine, University of Mousl - Iraq.
4. Boor K.J., Wiedmann M., Murphy S., Alcaine S. (2017). A 100-year review: microbiology and safety of milk handling. Journal of Dairy Science. 100: 9933-9951. [DOI: 10.3168/ jds.2017-12969] [DOI:10.3168/jds.2017-12969] [PMID]
5. Bouzid M., Kintz E., Hunter P.R. (2018). Risk factors for Cryptosporidium infection in low and middle income countries: a systematic review and meta-analysis. PLOS Neglected Tropical Diseases. 12: e0006553. [DOI: 10.1371/journal. pntd.0006553] [DOI:10.1371/journal.pntd.0006553] [PMID] [PMCID]
6. Cacciò S.M., Chalmers R.M. (2016). Human cryptosporidiosis in Europe. Clinical Microbiology and Infection. 22: 471-480. [DOI: 10.1016/j.cmi.2016.04.021] [DOI:10.1016/j.cmi.2016.04.021] [PMID]
7. Cai Y., Zhang N.-Z., Gong Q.-L., Zhao Q., Zhang X.-X. (2019). Prevalence of Cryptosporidium in dairy cattle in China during 2008-2018: a systematic review and meta-analysis. Microbial Pathogenesis. 132: 193-200. [DOI: 10.1016/j. micpath.2019.05.006] [DOI:10.1016/j.micpath.2019.05.006] [PMID]
8. Certad G., Viscogliosi E., Chabé M., Cacciò S.M. (2017). Pathogenic mechanisms of Cryptosporidium and Giardia. Trends in Parasitology. 33: 561-576. [DOI: 10.1016/j.pt. 2017.02.006] [DOI:10.1016/j.pt.2017.02.006] [PMID]
9. Chalmers R., Cacciò S. (2016). Towards a consensus on genotyping schemes for surveillance and outbreak investigations of Cryptosporidium, Berlin, June 2016. Eurosurveillance. 21: 30338. [DOI: 10.2807/1560-7917.ES.2016.21.37.30338] [DOI:10.2807/1560-7917.ES.2016.21.37.30338] [PMID] [PMCID]
10. Chauhan S., Powar P., Mehra R. (2021). A review on nutritional advantages and nutraceutical properties of cow and goat milk. International Journal of Applied Research. 7: 101-105. [DOI: 10.22271/allresearch.2021.v7.i10b.9025] [DOI:10.22271/allresearch.2021.v7.i10b.9025]
11. Dalimi A., Tahvildar F., Ghaffarifar F. (2017). Molecular study on Cryptosporidium andersoni strains isolated from sheep based on 18S rRNA gene. Infection, Epidemiology and Microbiology. 3: 100-103. [DOI: 10.18869/modares.iem.3.3.100]
12. EFSA BIOHAZ Panel (EFSA Panel on Biological Hazards). (2015). Scientific opinion on the public health risks related to the consumption of raw drinking milk. EFSA Journal. 13: 3940. [DOI: 10.2903/j.efsa.2015.3940]EFSA Panel on Biological Hazards (BIOHAZ), Koutsoumanis K., Allende A., lvarez-Ordóñez A., Bolton D., Bover-Cid S., Chemaly M., Davies R., De Cesare A., Herman L., Hilbert F., Lindqvist R., et al. (2018). Public health risks associated with food-borne parasites. EFSA Journal. 16: 5495. [DOI: 10. 2903/j.efsa.2018.5495] [DOI:10.2903/j.efsa.2015.3940]
13. European Centre for Disease Prevention and Control. (2018). Cryptosporidiosis. In: ECDC. Annual epidemiological report for 2016. Stockholm, December 2018.
14. Fayer R., Santín M., Xiao L. (2005). Cryptosporidium bovis n. sp. (apicomplexa: cryptosporidiidae) in cattle (Bos taurus). The Journal of Parasitology. 91: 624-629. [DOI: 10.1645/GE-3435] [DOI:10.1645/GE-3435] [PMID]
15. Fayer R., Xiao L. (2008). Cryptosporidium and cryptosporidiosis. 2nd edition. CRC Press, Boca Raton, London, New York. [DOI:10.1201/9781420052275]
16. Feng Y., Ortega Y., He G., Das P., Xu M., Zhang X, Fayer R., Gatei W., Cama V., Xiao L. (2007). Wide geographic distribution of Cryptosporidium bovis and the deer-like genotype in bovines. Veterinary Parasitology. 144: 1-9. [DOI: 10.1016/j.vetpar.2006.10.001] [DOI:10.1016/j.vetpar.2006.10.001] [PMID]
17. Harp J.A., Fayer R., Pesch B.A., Jackson G.J. (1996). Effect of pasteurization on infectivity of Cryptosporidium parvum oocysts in water and milk. Applied and Environmental Microbiology. 62: 2866-2868. [DOI: 10.1128/aem.62.8.2866-2868.1996] [DOI:10.1128/aem.62.8.2866-2868.1996] [PMID] [PMCID]
18. Harper C.M., Cowell N.A., Adams B.C., Langley A.J., Wohlsen T.D. (2002). Outbreak of Cryptosporidium linked to drinking unpasteurised milk. Communicable Diseases Intelligence Quarterly Report. 26: 449-450.
19. Hasan M.H., Al-Taee A.F., Al-Hubaety A.K., Alani A.J. (2018). Detection of Cryptosporidium oocysts in ovine and caprine raw milk in relation to rearing management. Assiut Veterinary Medical Journal. 64: 47-50. [DOI: 10.21608/ AVMJ.2018.168688] [DOI:10.21608/avmj.2018.168688]
20. Hawash Y. (2014). DNA extraction from protozoan oocysts/cysts in feces for diagnostic PCR. Korean Journal of Parasitology. 52: 263-271. [DOI: 10.3347/kjp.2014.52.3.263] [DOI:10.3347/kjp.2014.52.3.263] [PMID] [PMCID]
21. Institute of Standards and Industrial Research of Iran (ISIRI). (2008). Milk and milk products - guidance on sampling. 3rd revision. National Standard No. 326. URL: file:///C:/Users/ admin/Downloads/326.pdf.
22. Koyama Y., Satoh M., Maekawa K., Hikosaka K., Nakai Y. (2005). Isolation of Cryptosporidium andersoni Kawatabi type in a slaughterhouse in the northern island of Japan. Veterinary Parasitology. 130: 323-326. [DOI: 10.1016/j. vetpar.2005.03.014] [DOI:10.1016/j.vetpar.2005.03.014] [PMID]
23. Lal A., Cornish L.M., Fearnley E., Glass K., Kirk M. (2015). Cryptosporidiosis: a disease of tropical and remote areas in Australia. PLOS Neglected Tropical Diseases. 9: e0004078. [DOI: 10.1371/journal.pntd.0004078] [DOI:10.1371/journal.pntd.0004078] [PMID] [PMCID]
24. Lasprilla-Mantilla M.I., Wagner V., Pena J., Frechette A., Thivierge K., Dufour S., Fernandez-Prada C. (2019). Effects of recycled manure solids bedding on the spread of gastrointestinal parasites in the environment of dairies and milk. Journal of Dairy Science. 102: 11308-11316. [DOI: 10.3168/jds.2019-16866] [DOI:10.3168/jds.2019-16866] [PMID]
25. Lorenzo J.M., Munekata P.E., Dominguez R., Pateiro M., Saraiva J.A., Franco D. (2018). Main groups of microorganisms of relevance for food safety and stability: general aspects and overall description. Innovative Technologies for Food Preservation, Academic Press. pp: 53-107. [DOI: 10.1016/ B978-0-12-811031-7.00003-0] [DOI:10.1016/B978-0-12-811031-7.00003-0] [PMCID]
26. López Torres L.L., López Cuevas O., Vázquez Vázquez C., Alvarado Gómez O.G., Vázquez Alvarado R.E., Rodríguez Fuentes H., Chaidez Quiroz C., Vidales Contreras J.A. (2020). Prevalence of Cryptosporidium spp. in dairy livestock from the Laguna region, Mexico. Revista Bio Ciencias. 7: e881. [DOI: 10.15741/revbio.07.e881]
27. Machado E.C.L., Stamford T.L.M., Alves L.C., Melo R.G., Shinohara N.K.S. (2006). Effectiveness of Cryptosporidium spp. oocysts detection and enumeration methods in water and milk samples. Arquivo Brasileiro de Medicina Veterináriae Zootecnia. 58: 432-439. [DOI: 10.1590/S0102-09352006000300023] [DOI:10.1590/S0102-09352006000300023]
28. Melini F., Melini V., Luziatelli F., Ruzzi M. (2017). Raw and heat-treated milk: from public health risks to nutritional quality. Beverages. 3: 54. [DOI: 10.3390/beverages3040054] [DOI:10.3390/beverages3040054]
29. Messner M.J., Berger P. (2016). Cryptosporidium infection risk: results of new dose-response modeling. Risk Analysis. 36: 1969-1982. [DOI: 10.1111/risa.12541] [DOI:10.1111/risa.12541] [PMID]
30. Mirian S.J., Asadi M.R., Ferdowsi H.R., Rezakhani A.H. (2010). A survey on horse cryptosporidial infection in Tehran Province. Archives of Razi Institute. 65: 45-47. [DOI: 10.22092/ARI. 2010.103851]
31. Mosa A.I. (2016). Occurrence of Cryptosporidium species and Toxoplasma gondii as protozoan parasites in raw milk. Ph.D. Thesis. Faculty of Veterinary Medicine, Assiut University, Egypt.
32. Motavalli Haghi M., Etemadifar F., Fakhar M., Hosseini Teshnizi S., Soosaraei M., Shokri A., Hajihasani A., Mashhadi H. (2017). Status of babesiosis among domestic herbivores in Iran: a systematic review and meta-analysis. Parasitology Research. 116: 1101-1109. [DOI: 10.1007/s00436-016-5368-8] [DOI:10.1007/s00436-016-5368-8] [PMID]
33. Motavalli Haghi M., Khorshidvand Z., Khazaei S., Foroughi-Parvar F., Sarmadian H., Barati N., Etemadifar F., Ghasemikhah R. (2020). Cryptosporidium animal species in Iran: a systematic review and meta-analysis. Tropical Medicine and Health. 48: 97. [DOI: 10.1186/s41182-020-00278-9] [DOI:10.1186/s41182-020-00278-9] [PMID] [PMCID]
34. Nguyen S.T., Nguyen D.T., Le D.Q., Le Hua L.N., Van Nguyen T., Honma H., Nakai Y. (2007). Prevalence and first genetic identification of Cryptosporidium spp. in cattle in central Viet Nam. Veterinary Parasitology. 150: 357-361. [DOI: 10.1016/j.vetpar.2007.09.010] [DOI:10.1016/j.vetpar.2007.09.010] [PMID]
35. Oliver S.P., Jayarao B.M., Almeida R.A. (2005). Foodborne pathogens in milk and the dairy farm environment: food safety and public health implications. Foodborne Pathogens and Disease. 2. [DOI: 10.1089/fpd.2005.2.115] [DOI:10.1089/fpd.2005.2.115] [PMID]
36. Olson M.E., O'Handley R.M., Ralston B.J., McAllister T.A., Pumipuntu N., Piratae S. (2018). Cryptosporidiosis: a zoonotic disease concern. Veterinary World. 11: 681-686. [DOI: 10.14202/vetworld.2018.681-686] [DOI:10.14202/vetworld.2018.681-686] [PMID] [PMCID]
37. Rahimi E. (2013). Enterotoxigenicity of Staphylococcus aureus isolated from traditional and commercial dairy products marketed in Iran. Brazilian Journal of Microbiology. 44: 393-399. [DOI: 10.1590/S1517-83822013000200008] [DOI:10.1590/S1517-83822013000200008] [PMID] [PMCID]
38. Razavi S.M., Nasirinasab-Rafsanjani M., Bahrami S. (2010). A study on Cryptosporidium contamination in lettuce collected from different areas in Shiraz. Journal of Shahrekord University of Medical Sciences. 12: 44-50. [Persian with English abstract]
39. Rosenthal M., Pedersen R., Leibsle S., Hill V., Carter K., Roellig D.M. (2015). Cryptosporidiosis associated with consumption of unpasteurized goat milk - Idaho, 2014. Morbidity and Mortality Weekly Report. 64: 194-195.
40. Ryan U., Hijjawi N., Xiao L. (2018). Foodborne cryptosporidiosis. International Journal for Parasitology. 48: 1-12. [DOI: 10.1016/j.ijpara.2017.09.004] [DOI:10.1016/j.ijpara.2017.09.004] [PMID]
41. Schmid R. (2009). The untold story of milk. New Trends Publishing, Washington, DC.
42. Sevinc F., Irmak K., Sevinc M. (2003). The prevalence of Cryptosporidium parvum infection in the diarrhoiec and non- diarrhoeic calves. Revue de Médecine Vétérinaire. 154: 357-362.
43. Shakerian A., Sharafati-chaleshtori R., Karshenas A.A., Rahimi E. (2015). Molecular detection of Cryptosporidium parvum in cow's raw milk in Isfahan Province, 2013. Medical Laboratory Journal. 9: 83-88. [Persian with English abstract]
44. Sleman Ali H., Mageed S.N., Jahed Khaniki Gh.R., Shariatifar N., Yunesian M., Rezaeian M., Saleh K.Kh. (2018). Contamination of Cryptosporidium spp. oocysts in raw vegetables produced in Koya city, Iraq. Journal of Food Quality and Hazards Control. 5: 89-93. [DOI: 10.29252/ jfqhc.5.3.89] [DOI:10.29252/jfqhc.5.3.89]
45. Thomson S., Hamilton C.A., Hope J.C., Katzer F., Mabbott N.A., Morrison L.J., Innes E.A. (2017). Bovine cryptosporidiosis: impact, host-parasite interaction and control strategies. Veter- inary Research. 48: 42. [DOI: 10.1186/s13567-017-0447-0] [DOI:10.1186/s13567-017-0447-0] [PMID] [PMCID]
46. Trotz-Williams L.A., Martin D.S., Gatei W., Cama V., Peregrine A.S., Martin S.W., Nydam D.V., Jamieson F., Xiao L. (2006). Genotype and subtype analyses of Cryptosporidium isolates from dairy calves and humans in Ontario. Parasitology Research. 99: 346-352. [DOI: 10.1007/s00436-006-0157-4] [DOI:10.1007/s00436-006-0157-4] [PMID]
47. Ursini T., Moro L., Requena-Méndez A., Bertoli G., Buonfrate D. (2020). A review of outbreaks of cryptosporidiosis due to unpasteurized milk. Infection. 48: 659-663. [DOI: 10.1007/ s15010-020-01426-3] [DOI:10.1007/s15010-020-01426-3] [PMID]
48. Wang R., Zhang X., Zhu H., Zhang L., Feng Y., Jian F., Ning C., Qi M., Zhou Y., Fu K., Wang Y., Sun Y., et al. (2011). Genetic characterizations of Cryptosporidium spp. and Giardia duodenalis in humans in Henan, China. Experimental Parasitology. 127: 42-45. [DOI: 10.1016/j.exppara.2010.06.034] [DOI:10.1016/j.exppara.2010.06.034] [PMID]
49. Wang Y., Cao J., Chang Y., Yu F., Zhang S., Wang R., Zhang L. (2020). Prevalence and molecular characterization of Cryptosporidium spp. and Giardia duodenalis in dairy cattle in Gansu, northwest China. Parasite. 27: 62. [DOI: 10. 1051/parasite/2020058] [DOI:10.1051/parasite/2020058] [PMID] [PMCID]
50. Willis C., Jørgensen F., Aird H., Elviss N., Fox A., Jenkins C., Fenelon D., Sadler-Reeves L., McLauchlin J. (2018). An assessment of the microbiological quality and safety of raw drinking milk on retail sale in England. Journal of Applied Microbiology. 124: 535-546. [DOI: 10.1111/jam.13660]Wochner K.F., Becker-Algeri T.A., Colla E., Badiale-Furlong [DOI:10.1111/jam.13660] [PMID]
51. E., Drunkler D.A. (2018). The action of probiotic microorganisms on chemical contaminants in milk. Critical Reviews in Microbiology. 44: 112-123. [DOI: 10.1080/ 1040841X.2017.1329275] [DOI:10.1080/1040841X.2017.1329275] [PMID]

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