Volume 5, Issue 4 (December 2018)                   J. Food Qual. Hazards Control 2018, 5(4): 118-127 | Back to browse issues page

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Macieira A, Barros D, Vaz-Velho M, Pinheiro R, Fonseca S, Albano H et al . Effects of Lactobacillus plantarum Bacteriocinogenic Culture on Physicochemical, Microbiological, and Sensorial Characteristics of “Chouriço Vinha d´Alhos”, a Traditional Portuguese Sausage. J. Food Qual. Hazards Control. 2018; 5 (4) :118-127
URL: http://jfqhc.ssu.ac.ir/article-1-481-en.html
Universidade Católica Portuguesa, CBQF-Centro de Biotecnologia e Química Fina-Laboratório Associado, Escola Superior de Biotecnologia, Rua Arquiteto Lobão Vital 172, 4200-374 Porto, Portugal
Abstract:   (554 Views)
Background: “Chouriço Vinha d’Alhos” is a traditional fermented dry meat sausage from North of Portugal. The aim of this work was to evaluate the effects of a fresh and a lyophilized bioprotective Lactobacillus plantarum ST153Ch culture, on an industrial scale, on the physicochemical, microbiological, and sensorial characteristics of “Chouriço Vinha d’Alhos”.
Methods: “Chouriço Vinha d’Alhos” added with Lb. plantarum ST153Ch (fresh or lyophilized) were analyzed for the physicochemical, microbiological, and sensorial characteristics, over 90 days of storage at 4 ºC. All data were statistically analyzed using an ANOVA procedure by IBM SPSS Statistics v. 25.
Results: The results showed that there was no difference in the reduction of L. monocytogenes, without or with the addition of Lb. plantarum either fresh or lyophilized. There were no significant differences (p>0.05) in some analyzed physicochemical parameters of products added with fresh or lyophilized Lb. plantarum cultures over the 90 days of storage; but both, fresh and lyophilized cultures, influenced some of the tested physicochemical parameters.  
Conclusion: Considering no significant differences between application methodologies (fresh or lyophilized Lb. plantarum), industry might be able to choose the most suitable method according to their manufacturing process.

DOI: 10.29252/jfqhc.5.4.2

Full-Text [PDF 528 kb]   (215 Downloads)    
Type of Study: Original article | Subject: Special
Received: 18/07/01 | Accepted: 18/09/09 | Published: 18/12/28

1. Albano H., Oliveira M., Aroso R., Cubero N., Hogg T., Teixeira P. (2007a). Antilisterial activity of lactic acid bacteria isolated from ''Alheiras'' (traditional Portuguese fermented sausages): in situ assays. Meat Science. 76: 796-800. [DOI:10.1016/j.meatsci.2007.01.019] [PMID]
2. Albano H., Todorov S.D., Van Reenen C.A., Hogg T., Dicks L.M.T., Teixeira P. (2007b). Characterization of two bacteriocins produced by Pediococcus acidilactici isolated from "Alheira", a fermented sausage traditionally produced in Portugal. International Journal of Food Microbiology. 116: 239-247. [DOI:10.1016/j.ijfoodmicro.2007.01.011] [PMID]
3. Barbosa J., Borges S., Amorim M., Pereira M.J., Oliveira A., Pintado M.E., Teixeira P. (2015). Comparison of spray drying, freeze drying and convective hot air drying for the production of a probiotic orange powder. Journal of Functional Foods. 17: 340-351. [DOI:10.1016/j.jff.2015.06.001]
4. Barros D.B., Sobrosa C., Pinheiro R., Fonseca S., Vaz-Velho M. (2018). Effect of addition of a starter cultures on physicochemical and sensory properties of "Alheira", a smoked sausage-like product. Millenium. 2: 39-48. [DOI:10.29352/mill0207.03.00195]
5. Benito M.J., Martín A., Aranda E., Perez-Nevado F., Ruiz-Moyano S., Cordoba M.G. (2007). Characterization and selection of autochthonous lactic acid bacteria isolated from traditional Iberian dry-fermented salchichón and chorizo sausages. Journal of Food Science. 72: 193-201. [DOI:10.1111/j.1750-3841.2007.00419.x] [PMID]
6. Botsoglou N.A., Grigoropoulou S.H., Botsoglou E., Govaris A., Papageorgiou G. (2003). The effects of dietary oregano essential oil and α-tocopheryl acetate on lipid oxidation in raw and cooked turkey during refrigerated storage. Meat Science. 65: 1193-1200. [DOI:10.1016/S0309-1740(03)00029-9]
7. Briers Y., Klumpp J., Schuppler M., Loessner M.J. (2011). Genome sequence of Listeria monocytogenes Scott A, a clinical isolate from a food-borne listeriosis outbreak. Journal of Bacteriology. 193: 4284-4285. [DOI:10.1128/JB.05328-11] [PMID] [PMCID]
8. Casaburi A., Aristoy M.C., Cavella S., Di Monaco R., Ecolini D., Toldra F. (2007). Biochemical and sensory characteristics of traditional fermented sausages of Vallo di Diano (Southern Italy) as affected by the use of starter cultures. Meat Science. 76: 295-307. [DOI:10.1016/j.meatsci.2006.11.011] [PMID]
9. Delgado A., López F.N.A., Brito D., Peres C., Fevereiro P., Garrido-Fernández A. (2007). Optimum bacteriocin production by Lactobacillus plantarum 17.2b requires absence of NaCl and apparently follows a mixed metabolite kinetics. Journal of Biotechnology. 130: 193-201. [DOI:10.1016/j.jbiotec.2007.01.041] [PMID]
10. Essid I., Hassouna M. (2013). Effect of inoculation of selected Staphylococcus xylosus and Lactobacillus plantarum strains on biochemical, microbiological and textural characteristics of a Tunisian dry fermented sausage. Food Control. 32: 707-714. [DOI:10.1016/j.foodcont.2013.02.003]
11. Ferreira V., Barbosa J., Silva J., Vendeiro S., Mota A., Silva F., Monteiro M.J., Hogg T., Gibbs P., Teixeira P. (2007). Chemical and microbiological characterisation of "Salpicão de Vinhais" and "Chouriça de Vinhais": traditional dry sausages produced in the North of Portugal. Food Microbiology. 24: 618-623. [DOI:10.1016/j.fm.2006.12.007] [PMID]
12. Fonseca S., Cachaldora A., Gómez M., Franco I., Carballo J. (2013). Effect of different autochthonous starter cultures on the volatile compounds profile and sensory properties of Galician chorizo, a traditional Spanish dry fermented sausage. Food Control. 33: 6-14. [DOI:10.1016/j.foodcont.2013.01.040]
13. Franciosa I., Alessandria V., Dolci P., Rantsiou K., Cocolin L. (2018). Sausage fermentation and starter cultures in the era of molecular biology methods. International Journal of Food Microbiology. 279: 26-32. [DOI:10.1016/j.ijfoodmicro.2018.04.038] [PMID]
14. Hussein F.H., Razavi S.H., Emam-Djomeh Z. (2017). Physicochemical properties and sensory evaluation of reduced fat fermented functional beef sausage. Applied Food Biotechnology. 4: 93-102.
15. International Standards Organization (ISO). (1997). Meat and meat products-Determination of moisture content (reference method). Standard No. 1442.
16. International Standards Organization (ISO). (1998). Microbiology of food and animal feeding stuffs-Horizontal method for the enumeration of mesophilic lactic acid bacteria-Colony-count technique at 30 degrees. Standard No. 152141.
17. International Standards Organization (ISO). (1999). Microbiology of food and animal feeding stuffs-Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species)-Part 1: technique using Baird-Parker agar medium. Standard No. 6888-1.
18. International Standards Organization (ISO). (2001). Microbiology of food and animal feeding stuffs-Horizontal method for the enumeration of beta-glucuronidase-positive Escherichia coli- Part 2: colony-count technique at 44 degrees C using 5-bromo-4-chloro-3-indolyl beta-D-glucuronide. Standard No. 16649-2.
19. International Standards Organization (ISO). (2003). Microbiology of the food chain-Horizontal method for the detection and enumeration of Clostridium spp.- Part 1: enumeration of sulfite-reducing Clostridium spp. by colony-count technique. Standard No. 15213-1.
20. International Standards Organization (ISO). (2004). Microbiology of food and animal feeding stuffs-Horizontal method for the enumeration of presumptive Bacillus cereus-Colony-count technique at 30 degrees C. Standard No. 7932.
21. International Standards Organization (ISO). (2009). Animal and vegetable fats and oils-Determination of acid value and acidity. Standard No. 660.
22. International Standards Organization (ISO). (2014). Microbiology of food, animal feed and water-Preparation, production, storage and performance testing of culture media. Standard No. 11133.
23. International Standards Organization (ISO). (2016). Microbiology of the food chain-Method validation-Part 2: protocol for the validation of alternative (proprietary) methods against a reference method. Standard No. 16149-2.
24. International Standards Organization (ISO). (2017a). Microbiology of the food chain- Horizontal method for the detection and enumeration of Listeria monocytogenes and of Listeria spp.-Part 1: detection method. Standard No. 11290-1.
25. International Standards Organization (ISO). (2017b). Microbiology of the food chain-Horizontal method for the detection, enumeration and serotyping of Salmonella-Part 1: detection of Salmonella spp. Standard No. 6579-1.
26. International Standards Organization (ISO). (2017c). Microbiology of the food chain-Horizontal method for the detection of pathogenic Yersinia enterocolitica. Standard No. 10273.
27. International Standards Organization (ISO). (2017d). Animal and vegetable fats and oils-Determination of peroxide - Iodometric (visual endpoint determination). Standard No. 3960.
28. Jácome S.L., Fonseca S., Pinheiro R., Todorov S.D., Noronha L., Silva J., Gomes A., Pintado M., Morais A.M.M.B., Teixeira P., Vaz-Velho M. (2014). Effect of lactic acid bacteria on quality and safety of ready-to-eat sliced cured/smoked meat products. Chemical Engineering Transactions. 38: 403-408.
29. Kouakou P., Ghalfi H., Destain J., Dubois-Dauphin R., Evrard P., Thonart P. (2009). Effects of curing sodium nitrite additive and natural meat fat on growth control of Listeria monocytogenes by the bacteriocin-producing Lactobacillus curvatus strain CWBI-B28. Food Microbiology. 26: 623-628. [DOI:10.1016/j.fm.2009.04.007] [PMID]
30. Kouakou P., Sidibe D., Biego H.G. (2016). Combinations of lyophilized cell-adsorbed bacteriocin of Lactobacillus curvatus CWBI-B28 with organic acids or salts to control Listeria monocytogenes on smoked salmon stored at 4 °C in vacuum packages. International Journal of Agricultural and Food Research. 5: 13-20.
31. Lorenzo J.M., Gómez M., Fonseca S. (2014). Effect of commercial starter cultures on physicochemical characteristics, microbial counts and free fatty acid composition of dry-cured foal sausage. Food Control. 46: 382-389. [DOI:10.1016/j.foodcont.2014.05.025]
32. Mainar M.S. Xhaferi R., Samapundo S., Devlieghere F., Leroy F. (2016). Opportunities and limitations for the production of safe fermented meats without nitrate and nitrite using an antibacterial Staphylococcus sciuri starter culture. Food Control. 69: 267-274. [DOI:10.1016/j.foodcont.2016.04.056]
33. Mataragas M., Drosinos E.H., Tsakalidou E., Metaxopoulos J. (2004). Influence of nutrients on growth and bacteriocin production by Leuconostoc mesenteroides L124 and Lactobacillus curvatus L442. Antonie van Leeuwenhoek. 85: 191-198. [DOI:10.1023/B:ANTO.0000020291.01957.a2] [PMID]
34. Oliveira M., Ferreira V., Magalhães R., Teixeira P. (2018). Biocontrol strategies for Mediterranean-style fermented sausages. Food Research International. 103: 438-449. [DOI:10.1016/j.foodres.2017.10.048] [PMID]
35. Orihuel A., Bonacina J., Vildoza M.J., Bru E., Vignolo G., Saavedra L., Fadda S. (2018). Biocontrol of Listeria monocytogenes in a meat model using a combination of a bacteriocinogenic strain with curing additives. Food Research International. 107: 289-296. [DOI:10.1016/j.foodres.2018.02.043] [PMID]
36. Perez R.H., Zendo T., Sonomoto K. (2014). Novel bacteriocins from lactic acid bacteria (LAB): various structures and applications. Microbial Cell Factories. 13: S3. [DOI:10.1186/1475-2859-13-S1-S3] [PMID] [PMCID]
37. Simion A.M.C., Vizireanu C., Alexe P., Franco I., Carballo J. (2014). Effect of the use of selected starter cultures on some quality, safety and sensorial properties of Dacia sausage, a traditional Romanian dry-sausage variety. Food Control. 35: 123-131. [DOI:10.1016/j.foodcont.2013.06.047]
38. Singh S., Shalini R. (2016). Effect of hurdle technology in food preservation: a review. Critical Reviews in Food Science and Nutrition. 56: 641-649. [DOI:10.1080/10408398.2012.761594] [PMID]
39. Todorov S.D., Franco B.D.G.M, Wiid I.J. (2014). In vitro study of beneficial properties and safety of lactic acid bacteria isolated from Portuguese fermented meat products. Beneficial Microbes. 5: 351-366. [DOI:10.3920/BM2013.0030] [PMID]
40. Todorov S.D., Vaz-Velho M., de Melo Franco B.D.G., Holzapfel W.H. (2013). Partial characterization of bacteriocins produced by three strains of Lactobacillus sakei, isolated from salpicao, a fermented meat product from North-West of Portugal. Food Control. 30: 111-121. [DOI:10.1016/j.foodcont.2012.07.022]
41. Van Reenen C.A., Dicks L.M., Chikindas M.L. (1998). Isolation, purification and partial characterization of plantaricin 423, a bacteriocin produced by Lactobacillus plantarum. Journal of Applied Microbiology. 84: 1131-1137. [DOI:10.1046/j.1365-2672.1998.00451.x] [PMID]
42. Vaz-Velho M., Jácome S., Noronha L., Todorovc S., Fonseca S., Pinheiro R., Morais A., Silva J., Teixeira P. (2013). Comparison of antilisterial effects of two strains of lactic acid bacteria during processing and storage of a Portuguese salami-like product "Alheira". Chemical Engineering Transactions. 32: 1807-1812.
43. Verluyten J., Leroy F., De Vuyst L. (2004). Influence of complex nutrient source on growth of and curvacin a production by sausage isolate Lactobacillus curvatus LTH 1174. Applied and Environmental Microbiology. 70: 5081-5088. https://doi.org/10.1128/AEM.70.9.5081-5088.2004 [DOI:10.1128/AEM.70.8.4807-4813.2004] [PMID] [PMCID]

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