Volume 10, Issue 4 (December 2023)
J. Food Qual. Hazards Control 2023, 10(4): 226-234 |
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Ethics code: IR.AUSMT.REC.1401.173
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Sohraby S, Alian Samakkhah S, Tooryan F, Norian R, Panahi Z. Evaluation of Tetracycline and Enrofloxacin Residues in Bovine Milk in Tehran Utilizing ELISA and HPLC Methods. J. Food Qual. Hazards Control 2023; 10 (4) :226-234
URL: http://jfqhc.ssu.ac.ir/article-1-1103-en.html
URL: http://jfqhc.ssu.ac.ir/article-1-1103-en.html
Department of Food Hygiene, Faculty of Veterinary Medicine, Amol University of Special Modern Technologies (AUSMT), Amol, Iran , S.alian@ausmt.ac.ir
Abstract: (138 Views)
Background: Milk is regarded as one of the most sources of nutrition in the world and has a high value for individual's health. Milk is consumed by sensitive groups including pregnant women, older adults, and children. Therefore, the significance of antibiotic on human health makes it crucial to monitor their existence in food. This investigation aims to evaluate enrofloxacin and tetracycline in the raw and pasteurized milk in Tehran.
Methods: In this cross-sectional study, 112 raw and 112 pasteurized milks were accumulated in spring and winter from six reputable brands and traditional dairy stores in Tehran from December 2021 until May 2022 for six months, and antibiotic residues were examined by Immunosorbent Assay and High-Performance Liquid Chromatography techniques.
Results: The findings indicated that the prevalence of tetracycline in raw and pasteurized milk was 41.07 and 26.78% in spring and 85.71 and 35.71% in winter, respectively. The median concentration of tetracycline was 33.27 and 22.65 ppb in spring and 55.81 and 21.91 ppb in winter, respectively. The prevalence of enrofloxacin in raw milk and pasteurized milk samples were 33.92 and 14.28% in spring and 64.28 and 32.14% in winter, respectively. The median concentrations of enrofloxacin were 9.13 and 9.38 ppb in spring and 10.57 and 11.23 ppb in winter, respectively. The raw and pasteurized milk samples collected in winter had higher percentage of antibiotic residue in terms of enrofloxacin than samples collected in spring (p<0.05). Furthermore, the quantity of tetracycline antibiotic in raw milk was significantly higher in winter than in spring (p=0.002). However, the pasteurized milk fails to have significant difference between two seasons. The finding showed the tetracycline and enrofloxacin in all samples were less than 100 ppb (standard limit), and there is no significant difference with the standard limit.
Conclusion: Based on the obtained results, monitoring the antibiotic residues in milk, controlling and minimizing these residues for human health are regarded crucial.
DOI: 10.18502/jfqhc.10.4.14181
Methods: In this cross-sectional study, 112 raw and 112 pasteurized milks were accumulated in spring and winter from six reputable brands and traditional dairy stores in Tehran from December 2021 until May 2022 for six months, and antibiotic residues were examined by Immunosorbent Assay and High-Performance Liquid Chromatography techniques.
Results: The findings indicated that the prevalence of tetracycline in raw and pasteurized milk was 41.07 and 26.78% in spring and 85.71 and 35.71% in winter, respectively. The median concentration of tetracycline was 33.27 and 22.65 ppb in spring and 55.81 and 21.91 ppb in winter, respectively. The prevalence of enrofloxacin in raw milk and pasteurized milk samples were 33.92 and 14.28% in spring and 64.28 and 32.14% in winter, respectively. The median concentrations of enrofloxacin were 9.13 and 9.38 ppb in spring and 10.57 and 11.23 ppb in winter, respectively. The raw and pasteurized milk samples collected in winter had higher percentage of antibiotic residue in terms of enrofloxacin than samples collected in spring (p<0.05). Furthermore, the quantity of tetracycline antibiotic in raw milk was significantly higher in winter than in spring (p=0.002). However, the pasteurized milk fails to have significant difference between two seasons. The finding showed the tetracycline and enrofloxacin in all samples were less than 100 ppb (standard limit), and there is no significant difference with the standard limit.
Conclusion: Based on the obtained results, monitoring the antibiotic residues in milk, controlling and minimizing these residues for human health are regarded crucial.
DOI: 10.18502/jfqhc.10.4.14181
Keywords: Enrofloxacin, Tetracycline, Milk, Chromatography High Pressure Liquid, Enzyme-linked Immunosorbent Assay
Type of Study: Original article |
Subject:
Special
Received: 23/04/03 | Accepted: 23/09/05 | Published: 23/12/30
Received: 23/04/03 | Accepted: 23/09/05 | Published: 23/12/30
References
1. Aalipour F., Mirlohi M., Jalali M. (2013). Prevalence of antibiotic residues in commercial milk and its variation by season and thermal processing methods. International Journal of Environmental Health Engineering. 2: 41. [DOI: 10.4103/2277-9183.122429] [DOI:10.4103/2277-9183.122429]
2. Alimohammadi M., Ghale Askari S., Morgan Azghadi N., Taghavimanesh V., Mohammadimoghadam T., Bidkhori M., Gholizade A., Rezvani R., Mohammadi A.A. (2020). Antibiotic residues in the raw and pasteurized milk produced in northeastern Iran examined by the four-plate test (FPT) method. International Journal of Food Properties. 23: 1248-1255. [DOI: 10.1080/10942912.2020.1800032] [DOI:10.1080/10942912.2020.1800032]
3. Beck K.L., Coad J. (2017). Dairy product (calcium) consumption and iron nutrition. In: Watson R.R., Collier R.J., Preedy V.R. (Editors). Nutrients in dairy and their implications on health and disease. Academic Press, Cambridge, Massachusetts, United States. pp: 149-160. [DOI: 10.1016/B978-0-12-809762-5.00012-7] [DOI:10.1016/B978-0-12-809762-5.00012-7]
4. Chiesa L.M., DeCastelli L., Nobile M., Martucci F., Mosconi G., Fontana M., Castrica M., Arioli F., Panseri S. (2020). Analysis of antibiotic residues in raw bovine milk and their impact toward food safety and on milk starter cultures in cheese-making process. LWT. 131: 109783. [DOI: 10.1016/j.lwt.2020.109783] [DOI:10.1016/j.lwt.2020.109783]
5. Dabbagh Moghaddam A., Tayebi L., Falahatpisheh H., Mahmoudian M., Kowsari N., Akbarein H., Sabzikar A. (2014). Evaluation of the tetracycline residues in pasteurized milks distributed in Tehran by HPLC method. Scientific and Research Journal of Army University of Medical Sciences. 11: 318-323. [Iranian with English abstract]
6. Du B., Wen F., Zhang Y., Zheng N., Li S., Li F., Wang J. (2019). Presence of tetracyclines, quinolones, lincomycin and streptomycin in milk. Food Control. 100: 171-175. [DOI: 10.1016/j.foodcont.2019.01.005] [DOI:10.1016/j.foodcont.2019.01.005]
7. Kabrite S., Bou-Mitri C., EI Hayek Fares J., Hassan H.F., Matar Boumosleh J. (2019). Identification and dietary exposure assessment of tetracycline and penicillin residues in fluid milk, yogurt, and labneh: a cross-sectional study in Lebanon. Veterinary world. 12: 527-534. [DOI: 10.14202/vetworld.2019. 527-534] [DOI:10.14202/vetworld.2019.527-534] [PMID] [PMCID]
8. Kubicová Ľ., Predanocyová K., Kádeková Z. (2019). The importance of milk and dairy products consumption as a part of rational nutrition. Potravinarstvo Slovak Journal of Food Sciences. 13: 234-243. [DOI: 10.5219/1050] [DOI:10.5219/1050]
9. Kurjogi M., Issa Mohammad Y.H., Alghamdi S., Abdelrahman M., Satapute P., Jogaiah S. (2019). Detection and determination of stability of the antibiotic residues in cow's milk. Plos One. 14: e0223475. [DOI: 10.1371/journal.pone.0223475] [DOI:10.1371/journal.pone.0223475] [PMID] [PMCID]
10. Liu P., Wu Z., Cannizzo F.T., Mantegna S., Cravotto G. (2022). Removal of antibiotics from milk via ozonation in a vortex reactor. Journal of Hazardous Materials. 440: 129642. [DOI: 10.1016/j.jhazmat.2022.129642] [DOI:10.1016/j.jhazmat.2022.129642] [PMID]
11. Mahmoudi R., Amini K., Vahabzade M., Mir H., Vagef R. (2014a). Antibiotic residues in raw and pasteurized milk, Iran. Journal of Research and Health. 4: 884-889.
12. Mahmoudi R., Norian R., Ghajarbeygi P. (2014b). Survey of antibiotic residues in raw milk samples in Qazvin (2012). Journal of Inflammatory Diseases. 18: 45-52. [Iranian with English abstract]
13. Meklati F.R., Panara A., Hadef A., Meribai A., Ben-Mahdi M.H., Dasenaki M.E., Thomaidis N.S. (2022). Comparative assessment of antibiotic residues using liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) and a rapid screening test in raw milk collected from the North-Central Algerian dairies. Toxics. 10: 19. [DOI: 10.3390/toxics10010019] [DOI:10.3390/toxics10010019] [PMID] [PMCID]
14. Mesgari Abbasi M., Babaei H., Ansarin M., Nourdadgar A.-O.-S., Nemati M. (2011). Simultaneous determination of tetracyclines residues in bovine milk samples by solid phase extraction and HPLC-FL method. Advanced Pharmaceutical Bulletin. 1: 34-39. [DOI: 10.5681/apb.2011.005]
15. Mohammadzadeh Moghadam M., Amiri M., Ramezani Awal Riabi H., Ramezani Awal Riabi H. (2016). Evaluation of antibiotic residues in pasteurized and raw milk distributed in the South of Khorasan-e Razavi province, Iran. Journal of Clinical and Diagnostic Research. 10: FC31-FC35. [DOI: 10.7860/JCDR/2016/21034.9034] [DOI:10.7860/JCDR/2016/21034.9034] [PMID] [PMCID]
16. Motarjemi Y., Moy G.G., Jooste P.J., Anelich L.E. (2014). Milk and dairy products. In: Motarjemi Y., Lelieveld H. (Editors). Food safety management: a practical guide for the food industry. Academic Press, Cambridge, Massachusetts, United States. pp: 83-117. [DOI: 10.1016/B978-0-12-381504-0.00005-6] [DOI:10.1016/B978-0-12-381504-0.00005-6]
17. Moudgil P., Bedi J.S., Aulakh R.S., Gill J.P.S. (2019a). Antibiotic residues and mycotoxins in raw milk in Punjab (India): a rising concern for food safety. Journal of Food Science and Technology. 56: 5146-5151. [DOI: 10.1007/s13197-019-03963-8] [DOI:10.1007/s13197-019-03963-8] [PMID] [PMCID]
18. Moudgil P., Bedi J.S., Aulakh R.S., Gill J.P.S., Kumar A. (2019b). Validation of HPLC multi-residue method for determination of fluoroquinolones, tetracycline, sulphonamides and chloramphenicol residues in bovine milk. Food Analytical Methods. 12: 338-346. [DOI: 10.1007/s12161-018-1365-0] [DOI:10.1007/s12161-018-1365-0]
19. Pirsaheb M., Khamotian R., Dargahi A., Torabi S., Ghasem Zadeh A. (2014). The survey of antibiotic residues of milk in Iran. Journal of Jiroft University of Medical Sciences. 1: 94-105. [Iranian with English abstract]
20. Rassouli A., Abdolmaleki Z., Bokaee S., Kamkar A., Shams G.H.R. (2010). A cross-sectional study on oxytetracycline and tetracycline residues in pasteurized milk supplied in Tehran by an HPLC method. International Journal of Veterinary Research. 4: 1-3.
21. Ray P.R., Sen C. (2019). Biochemical residues in milk and milk products-a review. Indian Journal of Animal Health. 58: 145-152. [DOI:10.36062/ijah.58.2SPL.2019.145-152]
22. Roba H.M. (2023). Review on antimicrobial residue occurrence in cow milk and its public health importance in Ethiopia. Archives of Nutrition and Public Health. 5.
23. Schmerold I., Van Geijlswijk I., Gehring R. (2023). European regulations on the use of antibiotics in veterinary medicine. European Journal of Pharmaceutical Sciences. 189: 106473. [DOI: 10.1016/j.ejps.2023.106473] [DOI:10.1016/j.ejps.2023.106473] [PMID]
24. Sharifi S., Sohrabvandi S., Mofid V., Khanniri E., Khorshidian N., Esmaeili S., Shadnoush M., Hadizadeh M., Mortazavian A.M. (2021). Determination of lead level in pasteurized milk and dairy products consumed in Tehran and evaluation of associated health risk. International Journal of Cancer Management. 14: e115541. [DOI: 10.5812/ijcm.115541] [DOI:10.5812/ijcm.115541]
25. Virto M., Santamarina-García G., Amores G., Hernández I. (2022). Antibiotics in dairy production: where is the problem?. Dairy. 3: 541-564. [DOI: 10.3390/dairy3030039] [DOI:10.3390/dairy3030039]
26. Yao S., Shi Z., Cao P., Zhang L., Tang Y., Zhou P., Liu Z. (2023). A global survey of organophosphate esters and their metabolites in milk: occurrence and dietary intake via milk consumption. Journal of Hazardous Materials. 442: 130080. [DOI: 10.1016/j.jhazmat.2022.130080] [DOI:10.1016/j.jhazmat.2022.130080] [PMID]
27. Zeina K., Pamela A.K., Fawwak S. (2013). Quantification of antibiotic residues and determination of antimicrobial resistance profiles of microorganisms isolated from bovine milk in Lebanon. Food and Nutrition Sciences. 4: 1-9. [DOI: 10.4236/fns.2013.47A001] [DOI:10.4236/fns.2013.47A001]
28. Zhang X., Tang X., Yu J., Ye H., Zhao L. (2023). A novel carbon dots synthesized based on easily accessible biological matrix for the detection of enrofloxacin residues. Microchemical Journal. 190: 108690. [DOI: 10.1016/j.microc.2023.108690] [DOI:10.1016/j.microc.2023.108690]
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